Abstract

Purpose

Prevalence of skin cancer is rapidly increasing. There is a need for non-invasive biomarkers to assess efficacy of prevention strategies aiming at reduction of exposure to ultraviolet radiation (UVR). Recently, stratum corneum (SC) biomarkers were applied in various inflammatory skin diseases. Here, we explore their suitability as candidate biomarkers for UVR.

Material and methods

Twelve volunteers were exposed to a UVB-dose of 0.72 SED, three times a week, during three weeks. As candidate biomarkers, cis-isomers of urocanic acid (cUCA) and 25 immunological mediators were measured in the SC.

Results

Eight immunological markers significantly changed from baseline. Of them, IL-1RA/IL-1α and a placental growth factor (PIGF) showed gradual changes during UVR-exposure (p < 0.01 for linear trend). cUCA increased sharply already after the first exposure, however, reached a plateau in the second week.

Conclusions

SC represents a promising, non-invasive alternative to skin biopsy in detecting UVR-induced changes. cUCA is the marker of choice for assessment of single UVR-exposure; however, it is less suitable for cumulative UVR-dose. Immunological markers including IL-1RA/IL-1α and PIGF showed gradual changes, and therefore are convenient for monitoring chronic UVR-exposure. These candidate biomarkers might facilitate assessment of the efficacy of preventive measures in the workplace and general population.

中文翻译：

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用胶带剥离角质层，作为亚红剂量下紫外线辐射的生物标志物：一项针对人类志愿者的研究。

摘要

目的

皮肤癌的发病率正在迅速增加。需要非侵入性生物标记物以评估旨在减少暴露于紫外线辐射（UVR）的预防策略的有效性。最近，角质层（SC）生物标记物已用于各种炎症性皮肤病。在这里，我们探讨了它们作为UVR候选生物标志物的适用性。

材料与方法

十二名志愿者在三周内每周接受三次UVB剂量为0.72 SED的剂量。作为候选生物标志物，在SC中测量了尿酸（cUCA）的顺式异构体和25种免疫学介体。

结果

八个免疫标记与基线相比发生了显着变化。其中，IL-1RA /IL-1α和胎盘生长因子（PIGF）在UVR暴露期间显示逐渐变化（ 线性趋势p <0.01）。在第一次接触后，cUCA已经急剧增加，但是在第二周达到了稳定水平。

结论

SC在检测UVR引起的变化方面代表了一种有希望的，无创的皮肤活检替代方法。cUCA是评估单一UVR暴露的选择标记；但是，它不太适合累积UVR剂量。包括IL-1RA /IL-1α和PIGF在内的免疫标志物显示逐渐变化，因此便于监测慢性UVR暴露。这些候选生物标志物可能有助于评估工作场所和普通人群中预防措施的有效性。