Lactobacilli are reported to reduce weight gain, the mechanism has not been well elucidated. In this study mice received either normal-chow-diet or high–fat-diet (HFD), and drinking water with or without addition of Lactobacillus plantarums HT121 for 11 weeks. We found that HT121 decreased the body weight and improved glucose tolerance in high-fat fed mice. ELISA analysis showed HT121 decreased levels of LPS and inflammatory cytokines in serum, and increased short chain acids levels in feces. 16S rRNA sequencing indicated HT121 restored Firmicutes/Bacteroidetes ratio, PCOA, major phyla, genera of Akkermansia, lactobacillus, and Ruminococcus changed by HFD to the levels of normal group. Untargeted metabolomics analysis indicated HT121 reversed the levels of discriminant metabolites in liver, such as taurine, isoleucine, L-phenylalanine, methionine, and spermidine changed by HFD. Our results provide a new insight in prevention and treatment of obesity and type 2 diabetes.

据报道，乳酸杆菌可减轻体重增加，其机制尚未得到很好的阐明。在这项研究中，小鼠接受正常饮食或高脂饮食（HFD），并在有或没有添加植物乳杆菌HT121的情况下饮水，持续11周。我们发现HT121可以减轻高脂喂养小鼠的体重并改善其葡萄糖耐量。ELISA分析显示HT121降低了血清中LPS和炎性细胞因子的水平，并增加了粪便中的短链酸水平。16S rRNA测序表明HT121恢复了Firmicutes / Bacteroidetes比率，PCOA，主要门，阿克曼属，乳杆菌和Ruminococcus通过HFD更改为正常组的水平。非靶向代谢组学分析表明，HT121逆转了由HFD改变的肝脏中鉴别性代谢物的水平，例如牛磺酸，异亮氨酸，L-苯丙氨酸，蛋氨酸和亚精胺。我们的结果为肥胖和2型糖尿病的预防和治疗提供了新的见识。