Abstract It is still a very challenging work to develop new and more effective methods for detecting Hepatitis B virus (HBV). In this work, a new simple electrochemical biosensor with high sensitivity and stability was constructed for detecting HBV DNA by combining the electroactive Cu-MOF as a signal nanoprobe and multi-site matrix and electroreduced graphene oxide (ErGO) as signal amplification material. Cu-MOF exhibits good affinity to probe DNA through covalent attachment, which can further stabilize DNA probe. The probe DNA density and the hybridization efficiency of this electrochemical DNA biosensor was 9.48 × 1013 molecules cm−2 and 89.9%, respectively. Under optimal conditions, the electrochemical DNA biosensor had an excellent performance toward HBV DNA determination with a wide linear range of 50.0 fM to 10.0 nM, and a low limit of detection (LOD) of 5.2 fM, more sensitive than other reported electrochemical HBV DNA biosensors. Moreover, the new sensor was used to measure HBV DNA in human serum and urine samples with sound results. This work also provides some insights for the construction of other electrochemical DNA biosensors.

中文翻译：

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基于 ErGO 支持的 Cu-MOF 的高灵敏度和稳定的电化学 HBV DNA 生物传感器

摘要 开发新的、更有效的乙型肝炎病毒（HBV）检测方法仍然是一项非常具有挑战性的工作。在这项工作中，通过将电活性 Cu-MOF 作为信号纳米探针和多位点基质和电还原氧化石墨烯 (ErGO) 作为信号放大材料，构建了一种具有高灵敏度和稳定性的新型简单电化学生物传感器，用于检测 HBV DNA。Cu-MOF 通过共价连接对探针 DNA 表现出良好的亲和力，可以进一步稳定 DNA 探针。这种电化学DNA生物传感器的探针DNA密度和杂交效率分别为9.48×1013分子cm-2和89.9%。在最佳条件下，电化学 DNA 生物传感器对 HBV DNA 测定具有优异的性能，线性范围为 50.0 fM 至 10.0 nM，检测下限 (LOD) 为 5.2 fM，比其他报道的电化学 HBV DNA 生物传感器更灵敏。此外，新传感器用于测量人血清和尿液样本中的 HBV DNA，结果良好。这项工作也为构建其他电化学 DNA 生物传感器提供了一些见解。