The application of polypeptides in bio-interfaces and biosensors is of great interest because polypeptides are biocompatible and easy to design. A novel polymer nanocomposite was prepared by the electropolymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) with a newly designed polypeptide. The nanocomposite polypeptide doped PEDOT (PEDOT/PEP), with a 3D microporous network structure, large surface area and excellent antifouling ability, was utilized for the attachment of BRCA1 complementary oligonucleotides to construct a DNA biosensor. The fabricated DNA biosensor showed favorable selectivity (with a detection limit of 0.0034 pM) and high sensitivity. The biosensor was also capable of detecting the target DNA (BRCA1) in 1% (V/V) human serum samples. The combination of a conducting polymer PEDOT with an antifouling and biocompatible polypeptide demonstrates a new method for preparing electrochemical sensors, that are capable of detecting targets in complex biological samples without strong nonspecific protein adsorption.

多肽在生物界面和生物传感器中的应用引起了极大的兴趣，因为多肽具有生物相容性并且易于设计。通过将导电聚合物聚（3,4-乙撑二氧噻吩）（PEDOT）与新设计的多肽进行电聚合制备了一种新型的聚合物纳米复合材料。纳米复合多肽掺杂的PEDOT（PEDOT / PEP）具有3D微孔网络结构，较大的表面积和优异的防污能力，可用于BRCA1互补寡核苷酸的连接，以构建DNA生物传感器。制备的DNA生物传感器显示出良好的选择性（检测限为0.0034 pM）和高灵敏度。该生物传感器还能够检测1％（V / V）人类血清样品中的目标DNA（BRCA1）。