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Fluorescent detection of Cu (II) ions based on DNAzymatic cascaded cyclic amplification
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-07-13 , DOI: 10.1007/s00604-020-04430-4
Jingjing Tian 1, 2, 3 , Zaihui Du 1, 2, 3 , Longjiao Zhu 1, 2, 3 , Xiangli Shao 1, 2 , Xiangyang Li 4 , Wentao Xu 1, 2, 3
Affiliation  

A fluorescent biosensor based on the cascaded cyclic amplification-lighted copper nanoparticles has been developed, optimized, and validated. In the double-modular cascaded cyclic amplification, a DNAzymatic cyclic amplification unit transforms metal ion signal to specific DNA sequences, and a linear/exponential integrated amplification unit converts as-prepared DNA codes to identical thymine (T)-rich DNA templates. T-rich scaffolds can induce the generation of red fluorescent copper nanoparticles, with fluorescence emission at 625 nm upon the excitation at 340 nm, as signal vehicles for quantitative detection of metal ions. Copper ions, selected as the model target, could be detected in a wide linear range from 10 to 10 4 nM depending on the increased fluorescent intensity, and the detection limit is 5.6 ± 0.52 nM ( n = 3) within 40 min, which is 4 orders of magnitude lower than the limits set in drinking water. In the detection of Cu 2+ in real tap and lake water, the results between inductively coupled plasma mass spectrometry (ICP-MS) and our proposed biosensor were consistent, illustrating the practicability of the fabricated method. In summary, the established fluorescent biosensor compensates the deficiency of immunoassays failing to analyze metal ions, broadens ranges of biomarkers responding to cleaved DNAzymes, provides an open platform sensing different metal ions, and meets the increasing need for the ultrasensitive detection in the field of food safety, environmental monitoring, and medical diagnosis.

中文翻译:

基于 DNAzymatic 级联循环扩增的 Cu (II) 离子的荧光检测

已经开发、优化和验证了基于级联循环放大发光铜纳米粒子的荧光生物传感器。在双模块级联循环扩增中,DNAzymatic 循环扩增单元将金属离子信号转换为特定的 DNA 序列,线性/指数集成扩增单元将制备的 DNA 代码转换为相同的富含胸腺嘧啶 (T) 的 DNA 模板。富含 T 的支架可以诱导红色荧光铜纳米粒子的产生,在 340 nm 激发时发出 625 nm 的荧光,作为金属离子定量检测的信号载体。选择作为模型目标的铜离子可以在 10 到 10 4 nM 的宽线性范围内检测到,这取决于荧光强度的增加,并且在 40 分钟内检测限为 5.6 ± 0.52 nM (n = 3),这比饮用水中设定的限值低 4 个数量级。在实际自来水和湖水中的Cu 2+ 检测中,电感耦合等离子体质谱(ICP-MS)与我们提出的生物传感器的结果一致,说明了该方法的实用性。总之,所建立的荧光生物传感器弥补了免疫分析无法分析金属离子的不足,拓宽了响应裂解脱氧核糖核酸酶的生物标志物范围,提供了一个感知不同金属离子的开放平台,满足了食品领域对超灵敏检测日益增长的需求。安全、环境监测和医疗诊断。电感耦合等离子体质谱(ICP-MS)和我们提出的生物传感器之间的结果是一致的,说明了所制造方法的实用性。总之,所建立的荧光生物传感器弥补了免疫分析无法分析金属离子的不足,拓宽了响应裂解脱氧核糖核酸酶的生物标志物范围,提供了一个感知不同金属离子的开放平台,满足了食品领域对超灵敏检测日益增长的需求。安全、环境监测和医疗诊断。电感耦合等离子体质谱(ICP-MS)和我们提出的生物传感器之间的结果是一致的,说明了所制造方法的实用性。总之,所建立的荧光生物传感器弥补了免疫分析无法分析金属离子的不足,拓宽了响应裂解脱氧核糖核酸酶的生物标志物范围,提供了一个感知不同金属离子的开放平台,满足了食品领域对超灵敏检测日益增长的需求。安全、环境监测和医疗诊断。
更新日期:2020-07-13
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