Four genotypes of soybean viz. NRC-45, IC-18734, J-231 and G-2132, which differ in their inherent ability to tolerate iron limiting stress, were selected as representative genotypes for iron efficient and responsive (FeER), iron inefficient and responsive (FeIR), iron efficient and nonresponsive (FeENR) and iron inefficient and nonresponsive (FeINR) categories, respectively. The relative performance of these genotypes to iron limiting stress was evaluated in hydroponics experiment with low iron (−Fe) and sufficient iron (+Fe). We hypothesised that the genotype exhibiting higher iron deficiency tolerance would exhibit greater root exudation capacity, which in turn can be screened through radiocarbon labelling technique, by counting the 14C content in the total root exudates (14CTRE). The genotype FeER (NRC-45) exuded out significantly higher amount of 14C in the total root exudates with the lower and upper bound values within 95% confidence interval (CI) were 151.9 and 163.6 Bq g−1 fw root−1 (FeER: NRC-45), followed by 138.5 and 150.1 Bq g−1 fw root−1 (FeENR: J-231), 86.5 and 98.1 Bq g−1 fw root−1 (FeIR: IC-18734), and 63.3 and 74.9 Bq g−1 fw root−1 (FeINR: G-2132) under Fe deficient (−Fe) condition.

中文翻译：

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使用根系分泌物中 14C 含量作为放射性碳标记筛选大豆缺铁耐受性的新应用的证据 (Glycine max (L.) Merr.) 基因型

大豆的四种基因型。NRC-45、IC-18734、J-231 和 G-2132，它们在耐受铁限制应力的固有能力方面不同，被选为铁高效和响应 (FeER)、铁低效和响应 (FeIR) 的代表性基因型，铁有效和无反应 (FeENR) 和铁无效和无反应 (FeINR) 类别，分别。在具有低铁 (-Fe) 和充足铁 (+Fe) 的水培实验中评估了这些基因型对铁限制应力的相对性能。我们假设表现出更高缺铁耐受性的基因型将表现出更大的根系分泌物能力，这反过来可以通过放射性碳标记技术通过计算总根系分泌物 (14CTRE) 中的 14C 含量来筛选。