Abstract

The complexes {[ZnL¹Cl] C1, [ZnL²Cl].0.5H₂O C2, [CdL¹Cl] C3, and [CdL²Cl] C4} were prepared from tridentate thiosemicarbazones {HL¹ = 4-(3-nitrophenyl)-1-((pyridin-2-yl)methylene) thiosemicarbazide and HL² = 4-(2,4-dimethoxyphenyl)-1-((pyridin-2-yl)methylene)thiosemicarbazide} and identified by elemental CHNS, spectroscopic {IR and UV–Vis.}, thermal and DMF solution electrical conductivity data. On another hand, kojic acid (KA) which represents important secondary metabolite with numerous hot spot applications was successfully biosynthesized from Aspergillus flavus and structurally analyzed by single crystal analysis. The Zn(II) complexes C1&C2 (0.3 mM) enhanced the KA biosynthesis by 70.87% and 42.26%, while 76.09% of C1 and 72.78% of C2 were absorbed by the fungal cells. The Cd(II) complexes C3&C4 at 0.3 mM inhibited KA production by 87.95% and 97.03% with Cd(II) consumption reaching to 40.09% & 37.3%, while 0.4 mM of C3&C4 resulted in 100% inhibition of kojic acid biosynthesis. Light microscopic analysis showed the fungal structural abnormalities and the cell antioxidant behavior was detected. These complexes could be highly applicable as new stimulators and inhibitors of kojic acid production.

Graphic abstract

中文翻译：

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Zn（II）和Cd（II）硫代半氨基甲酮类化合物可刺激/抑制黄曲霉的曲酸生物合成，以及针对金属配合物过量的真菌防御行为。

摘要

配合物{[ZnL ¹ Cl] C1，[ZnL ² Cl] .0.5H ₂ O C2，[CdL ¹ Cl] C3和[CdL ² Cl] C4 }是由三齿硫半咔唑{HL ¹  = 4-（3 -硝基苯基）-1-（（吡啶-2-基）亚甲基）硫代氨基脲和HL ²  = 4-（2,4-二甲氧基苯基）-1-（（吡啶-2-基）亚甲基）硫代氨基脲}并通过元素CHNS鉴定，光谱{IR和UV-Vis。}，热和DMF解决方案的电导率数据。另一方面，成功地通过以下方法成功地合成了代表重要次生代谢物且具有许多热点应用的曲酸（KA）：黄曲霉菌并通过单晶分析进行结构分析。Zn（II）配合物C1＆C2（0.3 mM）增强了KA生物合成70.87％和42.26％，而真菌细胞吸收了76.09％的C1和72.78％的C2。0.3 mM的Cd（II）复合物C3＆C4抑制KA产生87.95％和97.03％，Cd（II）消耗达到40.09％和37.3％，而0.4 mM的C3＆C4导致100％的曲酸生物合成受到抑制。光学显微镜分析显示真菌结构异常，并检测到细胞抗氧化行为。这些配合物可作为曲酸生产的新刺激剂和抑制剂而高度适用。

图形摘要