Antagonistic Plant growth promoting rhizobacteria can contribute to disease management by induction of host defenses and biological control of pathogens. The present study was thus planned to develop a bio-inoculant with the ability to promote plant growth and reduce disease incidence due to Fusarium oxysporum sp. ciceris in chickpea. Isolates of Mesorhizobium sp. (6) and endophytic bacteria (5) were screened as potential antagonists exhibiting 50–93.7% growth inhibition of Fusarium wilt (in vitro). Selected Mesorhizobium sp. and endophytic bacteria were assessed for their compatibility to develop dual combinations with no mutual inhibition. Dual inoculants significantly enhanced in vitro plant growth promoting traits viz., Indole Acetic Acid, siderophores, phosphate solubilization and 1-aminocyclopropane-1-carboxylate-deaminase enzyme activity. Dual mesorhizobia and endophytic bio-inoculants (able to grow with recommended fungicide captan @ 3 g/kg seed) were further assessed in wilt-sick field conditions for growth, symbiosis, soil health, yield and bio-control parameters in two chickpea varieties (PBG-7 and PBG-1). Seed priming with dual inoculants significantly enhanced growth, symbiosis, soil quality parameters and antioxidant enzymes viz., catalase, peroxidase and total phenols over control. Dual bio-inoculant LGR2 + LCNE8 improved the grain yield by 6.5% over captan as the control. These results suggest that bio-inoculants of Mesorhizobium sp. and endophytic bacteria under a competitive environment can be explored as bio-fertilizers/bio-protectants for improving productivity and suppression of Fusarium wilt in chickpea.

拮抗植物生长的根际细菌可以通过诱导宿主防御和对病原体的生物控制来促进疾病的防治。因此，本研究计划开发一种能够促进植物生长并减少由于尖孢镰刀菌引起的疾病发生的生物孕育剂。鹰嘴豆中的ciceris。分离的慢生根瘤菌属。（6）和内生细菌（5）被筛选为对枯萎病（体外）生长抑制50-93.7％的潜在拮抗剂。选择的中生根菌 和内生细菌的兼容性进行评估，以发展双重组合，没有相互抑制。双重接种剂显着增强了体外植物生长促进性状，即吲哚乙酸，铁载体，磷酸盐增溶和1-氨基环丙烷-1-羧酸酯脱氨酶活性。在枯萎病田间条件下进一步评估了两种中生根瘤菌和内生生物接种剂（能够与推荐的杀菌剂captan @ 3 g / kg种子一起生长）在两种鹰嘴豆品种中的生长，共生，土壤健康，产量和生物防治参数（ PBG-7和PBG-1）。用双重接种剂引发种子可显着增强生长，共生，土壤质量参数和抗氧化酶。，过氧化氢酶，过氧化物酶和总酚超标。与对照相比，双重生物杀菌剂LGR2 + LCNE8使谷物产量提高了6.5％。这些结果表明，慢生根瘤菌属。竞争环境下的内生细菌可以作为生物肥料/生物保护剂用于提高鹰嘴豆的生产力和抑制枯萎病。