A method for preparing a metal–organic framework (MOF)–polymeric monolith was established by in situ radical polymerization with derivatized UiO-66-2COOH and N-isopropylacrylamide (NIPAAm) as co-monomers. It was worth noting that the obtained monolith possessed characteristics of relatively uniform porous structure and a high surface area (84.16 m2 g−1). The adsorption capacity of the monolith increased from 22.87 to 44.92 mg−1 due to the addition of MOF. The monolith was used as adsorbent and coupled with HPLC for the online purification and extraction of ursolic acid (UA) in medicinal plant. The limit of detection (LOD, S/N = 3) and the limit of quantification (LOQ, S/N = 10) were 0.17 μg mL−1 and 0.57 μg mL−1, respectively; the relative standard deviation (RSD) of intra-day and inter-day assays for five plant samples were 1.00–3.67% and 2.43–5.76%, respectively; a good accuracy was demonstrated by the recovery of 92.2–103.28%. The results indicated that the MOF–polymer monolith was feasible for the online enrichment and purification of UA in plant.

中文翻译：

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以UiO-66-2COOH MOF为单体制备的整体柱用于在线固相萃取富集和纯化植物中的熊果酸

以衍生化的 UiO-66-2COOH 和 N-异丙基丙烯酰胺 (NIPAAm) 作为共聚单体，通过原位自由基聚合建立了一种制备金属有机骨架 (MOF) 聚合物整体的方法。值得注意的是，获得的整料具有相对均匀的多孔结构和高表面积（84.16 m2 g-1）的特性。由于添加了 MOF，整料的吸附容量从 22.87 增加到 44.92 mg-1。该整料用作吸附剂，并与 HPLC 联用，用于药用植物中熊果酸 (UA) 的在线纯化和提取。检测限 (LOD, S/N = 3) 和定量限 (LOQ, S/N = 10) 分别为 0.17 μg mL-1 和 0.57 μg mL-1；五个植物样品的日内和日间测定的相对标准偏差 (RSD) 为 1.00-3.67% 和 2.43-5。分别为 76%；92.2-103.28% 的回收率证明了良好的准确性。结果表明，MOF-聚合物整料可用于植物中UA的在线富集和纯化。