Bioherbicides afford satisfactory approach to overcome weed problem. Selection of new bioherbicides from phytopathogenic microbiota is essential for modern agricultural management, especially mycoherbicides. Thus, in the present investigation, 4 phytopathogenic fungi including Alternaria alternata YMM1, A. tenuissima YMM3, Chaetomium globosum YMM2, and Nigrospora oryzae YMM4 were isolated from infected leaves with chlorotic rings of Rumex dentatus and Sonchus oleraceus as common weeds in Egypt. These fungi were identified based on morphology and sequence analysis of ITS1-5.8SrDNA-ITS2 of rRNA genes. Mycoherbicidal metabolites were produced using submerged cultures in potato dextrose broth. Their bioherbicidal activities were evaluated using seed germination and leaf disk puncture assays. It was found that the most promising fungal strain against major tested weeds was N. oryzae YMM4. Thus, phytopathogenic microbiota are considered a great resource for the construction of a new bioherbicide for managing weeds.

中文翻译：

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植物病原性真菌分离物作为抗某些杂草的生物防治剂的潜力

生物除草剂提供了克服杂草问题的令人满意的方法。从植物致病菌群中选择新的生物除草剂对于现代农业管理至关重要，尤其是抗除草剂。因此，在本研究中，从埃及常见杂草的带有齿状轮生齿兰（Rumex dentatus）和油桐（Sonchus oleraceus）的含氯环上分离出4种植物致病真菌，其中包括链格孢（Alternaria alternata）YMM1，A。tenuissima YMM3，球壳拟杆菌（Chaetomium globosum）YMM4和稻瘟病菌（Ygrospora oryzae YMM4）。根据rRNA基因的ITS1-5.8SrDNA-ITS2的形态和序列分析鉴定了这些真菌。使用马铃薯葡萄糖肉汤中的浸没培养物产生杀真菌的代谢产物。使用种子发芽和叶盘穿刺试验评估了它们的杀生物活性。发现针对主要测试杂草的最有希望的真菌菌株是稻瘟病菌YMM4。因此，植物病原微生物群被认为是构建用于治理杂草的新型生物除草剂的重要资源。