Abstract Xylan deposition and lignification processes were examined in tension wood fibers with gelatinous layers (G-layers) in Mallotus japonicus (Euphorbiaceae). The cell walls consisted of a multi-layered structure of S1 + S2 + G + n(L + G), where n indicates the number of repetitions (n = 0–3) and L indicates very thin lignified layers. The formation and lignification processes of the multi-layered structure of tension wood fibers were examined by light microscopy, ultraviolet microscopy, and transmission electron microscopy (TEM) following KMnO4 staining. The deposition of xylan was examined by immunoelectron microscopy with a monoclonal antibody (LM11). Immunolabelling of xylan appeared in lignified cell wall layers, except in the compound middle lamella (CML), i.e., the S1, S2, and L layers but not the G-layers. The density of LM11 xylan immunogold labeling in S2 layers increased during the formation of G-layers. This increase was due to the shrinkage of S2 layers during development rather than intrusive deposition of xylan through G-layers. Lignification of the CML, S1, and S2 layers proceeded during G-layer formation. The shrinkage of S2 layers occurred almost simultaneously with the lignification of the S2 layers during G-layer formation, suggesting that the S2 layers shrank with lignification.

中文翻译：

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木聚糖沉积和木质化在区分具有多层结构的大戟科植物张力木纤维中的作用

摘要 木聚糖沉积和木质化过程在具有胶状层（G 层）的木纹木（大戟科）的张力木纤维中进行了研究。细胞壁由 S1 + S2 + G + n(L + G) 的多层结构组成，其中 n 表示重复次数 (n = 0–3)，L 表示非常薄的木质化层。在 KMnO4 染色后，通过光学显微镜、紫外线显微镜和透射电子显微镜 (TEM) 来检查张力木纤维多层结构的形成和木质化过程。木聚糖的沉积用单克隆抗体 (LM11) 通过免疫电子显微镜检查。木聚糖的免疫标记出现在木质化的细胞壁层中，除了复合中间层 (CML)，即 S1、S2 和 L 层，而不是 G 层。在 G 层形成过程中，S2 层中 LM11 木聚糖免疫金标记的密度增加。这种增加是由于开发过程中 S2 层的收缩，而不是木聚糖通过 G 层的侵入性沉积。CML、S1 和 S2 层的木质化在 G 层形成过程中进行。在 G 层形成过程中，S2 层的收缩几乎与 S2 层的木质化同时发生，表明 S2 层随着木质化而收缩。