This study investigated the effect of pentoxifylline (PTX) and Basal Medium Eagle (BME) on frozen–thawed goat spermatozoa. Immediately after initial examination of ejaculated semen, samples were pooled and reexamined for quality. Then, samples were divided into eight equal aliquots and diluted with a basic tris‐extender containing PTX (3, 6, 9 mM) and BME (5 mM) to reach a final concentration of 25 × 10⁹ and frozen. After 24 hr, the samples were individually thawed at 37°C for 30 s and evaluated for different characteristics. Obtained post‐thaw results from Computer‐Assisted Sperm Analysis indicate using of 3 and 6 mM PTX led significantly to an improvement in total motility, progressive motility and velocity characteristics of spermatozoa, except the beat/cross frequency (BCF) which indicated statistically no differences (p > .05) among control and treatments. Diluents prepared with BME (5 mM) and PTX alone (3 and 6 mM) improved significantly the membrane integrity–functionality, acrosome integrity and also hyaluronidase activity. Regarding recovery rate, the results showed significantly (p < .05) higher values for diluents containing 3 and 6 mM PTX compared to other groups. Malondialdehyde concentration exhibited also a significant difference (p < .05) in diluents supplemented with 5 mM BME, 3, 6 and 9 mM PTX, and mixture of 3 mM PTX and 5 mM BME which illustrate a similarity for active mitochondria, apoptotic‐like and dead spermatozoa. Finally, the ratio of sperm chromatin dispersion stained spermatozoa presented significant differences (p < .05) among treatments in which the diluents added PTX alone demonstrated significantly lower values than control and extenders containing the mixtures of BME and PTX. In conclusion, the observation in this study indicates using of 3 and 6 mM PTX and BME alone may improve significantly (p < .05) the quality of cryopreserved goat spermatozoa.

中文翻译：

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己酮可可碱和基础培养基 Eagle 补充稀释剂对冷冻保存的山羊精子的评价。

本研究调查了己酮可可碱 (PTX) 和基础培养基鹰 (BME) 对冻融山羊精子的影响。在对射出的精液进行初步检查后，立即收集样本并重新检查质量。然后，将样品分成八等份，并用含有 PTX (3, 6, 9 mM) 和 BME (5 mM) 的碱性 tris-extender 稀释，以达到 25 × 10 ⁹的终浓度和冻结。24 小时后，将样品在 37°C 下单独解冻 30 秒，并评估不同的特性。从计算机辅助精子分析获得的解冻后结果表明，使用 3 和 6 mM PTX 显着改善了精子的总运动性、渐进性运动性和速度特征，但搏动/交叉频率 (BCF) 表明统计学上没有差异( p  > .05) 在对照和处理之间。用 BME (5 mM) 和单独的 PTX（3 和 6 mM）制备的稀释剂显着改善了膜完整性 - 功能、顶体完整性和透明质酸酶活性。关于回收率，结果显示显着（p < .05) 与其他组相比，含有 3 和 6 mM PTX 的稀释剂的值更高。丙二醛浓度 在补充有 5 mM BME、3、6 和 9 mM PTX 以及 3 mM PTX 和 5 mM BME 的混合物的稀释剂中也表现出显着差异（p < .05），这说明活性线粒体的相似性，凋亡样和死精子。最后，精子染色质分散染色精子的比率 在单独添加 PTX 的稀释剂显示出显着低于包含 BME 和 PTX 混合物的对照和增量剂的处理之间存在显着差异 ( p < .05)。总之，本研究中的观察表明，单独使用 3 和 6 mM PTX 和 BME 可能会显着改善（p < .05) 冷冻保存的山羊精子的质量。