Pseudomonas glycinae sp. nov. isolated from the soybean rhizosphere.,MicrobiologyOpen

当前位置： X-MOL 学术 › Microbiologyopen › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Pseudomonas glycinae sp. nov. isolated from the soybean rhizosphere.
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-07-12 , DOI: 10.1002/mbo3.1101
Jiayuan Jia ₁ , Xiaoqiang Wang _{1,

2} , Peng Deng ₁ , Lin Ma _{1,

3} , Sonya M Baird ₁ , Xiangdong Li ₄ , Shi-En Lu ₁

Affiliation

Strains MS586^T and MS82, which are aerobic, Gram‐negative, rod‐shaped, and polar‐flagellated bacteria, were isolated from the soybean rhizosphere in Mississippi. Taxonomic positions of MS586^T and MS82 were determined using a polyphasic approach. 16S rRNA gene sequence analyses of the two strains showed high pairwise sequence similarities (>98%) to some Pseudomonas species. Analysis of the concatenated 16S rRNA, rpoB, rpoD, and gyrB gene sequences indicated that the strains belonging to the Pseudomonas koreensis subgroup (SG) shared the highest similarity with Pseudomonas kribbensis strain 46‐2^T. Analyses of average nucleotide identity (ANI), genome‐to‐genome distance, delineated MS586^T and MS82 from other species within the genus Pseudomonas. The predominant quinone system of the strain was ubiquinone 9 (Q‐9), and the DNA G+C content was 60.48 mol%. The major fatty acids were C_16:0, C_17:0 cyclo, and the summed features 3 and 8 consisting of C_16:1ω7c/C_16:1ω6c and C_18:1ω7c/C_18:1ω6c, respectively. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. Based on these data, it is proposed that strains MS586^T and MS82 represent a novel species within the genus Pseudomonas. The proposed name for the new species is Pseudomonas glycinae, and the type strain is MS586^T (accession NRRL B‐65441 = accession LMG 30275).

中文翻译：

大豆假单胞菌 sp.十一月从大豆根际分离。

菌株 MS586 ^T和 MS82 是需氧、革兰氏阴性、杆状、极鞭毛细菌，从密西西比州的大豆根际分离出来。使用多相方法确定 MS586 ^T和 MS82 的分类位置。两个菌株的 16S rRNA 基因序列分析显示与某些假单胞菌属物种具有高度的成对序列相似性 (>98%)。对串联的 16S rRNA、 rpoB 、 rpoD和gyrB基因序列的分析表明，属于朝鲜假单胞菌亚组 (SG) 的菌株与克里宾假单胞菌菌株 46-2 ^T具有最高的相似性。对平均核苷酸同一性 (ANI)、基因组间距离的分析，将 MS586 ^T和 MS82 与假单胞菌属内的其他物种分开。该菌株的优势醌系统为泛醌9（Q-9），DNA G+C含量为60.48 mol%。主要脂肪酸是C _16:0 、C _17:0环，并且总和特征3和8分别由C _16:1 ω7c/C _16:1 ω6c和C _18:1 ω7c/C _18:1 ω6c组成。主要的极性脂质是磷脂酰甘油、磷脂酰乙醇胺和二磷脂酰甘油。基于这些数据，建议菌株 MS586 ^T和 MS82 代表假单胞菌属内的新物种。新物种的拟定名称为Pseudomonas giginae ，模式菌株为 MS586 ^T （登录号 NRRL B-65441 = 登录号 LMG 30275）。

更新日期：2020-07-12

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11