Strains MS586^T and MS82, which are aerobic, Gram‐negative, rod‐shaped, and polar‐flagellated bacteria, were isolated from the soybean rhizosphere in Mississippi. Taxonomic positions of MS586^T and MS82 were determined using a polyphasic approach. 16S rRNA gene sequence analyses of the two strains showed high pairwise sequence similarities (>98%) to some Pseudomonas species. Analysis of the concatenated 16S rRNA, rpoB, rpoD, and gyrB gene sequences indicated that the strains belonging to the Pseudomonas koreensis subgroup (SG) shared the highest similarity with Pseudomonas kribbensis strain 46‐2^T. Analyses of average nucleotide identity (ANI), genome‐to‐genome distance, delineated MS586^T and MS82 from other species within the genus Pseudomonas. The predominant quinone system of the strain was ubiquinone 9 (Q‐9), and the DNA G+C content was 60.48 mol%. The major fatty acids were C_16:0, C_17:0 cyclo, and the summed features 3 and 8 consisting of C_16:1ω7c/C_16:1ω6c and C_18:1ω7c/C_18:1ω6c, respectively. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. Based on these data, it is proposed that strains MS586^T and MS82 represent a novel species within the genus Pseudomonas. The proposed name for the new species is Pseudomonas glycinae, and the type strain is MS586^T (accession NRRL B‐65441 = accession LMG 30275).

中文翻译：

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甘蔗假单胞菌 十一月 从大豆根际中分离出来。

从密西西比州的大豆根际中分离出好氧，革兰氏阴性，杆状和极鞭毛细菌MS586 ^T和MS82。MS586 ^T和MS82的分类位置使用多相方法确定。两种菌株的16S rRNA基因序列分析显示与某些假单胞菌属物种具有很高的成对序列相似性（> 98％）。对串联的16S rRNA，rpoB，rpoD和gyrB基因序列的分析表明，属于韩国假单胞菌（SG）的菌株与假单胞菌46-2 ^T菌株具有最高的相似性。对假单胞菌属中其他物种的平均核苷酸同一性（ANI），基因组到基因组的距离进行了分析，描绘出了MS586 ^T和MS82 。该菌株的主要醌系统是泛醌9（Q-9），DNA G + C含量为60.48 mol％。主要脂肪酸为C _16：0，C _17：0环，且总和3和8分别由C _16： 1ω7c/ C _16： 1ω6c和C _18： 1ω7c/ C _18： 1ω6c组成。主要的极性脂质是磷脂酰甘油，磷脂酰乙醇胺和二磷脂酰甘油。基于这些数据，建议菌株MS586 ^TMS82和MS82代表假单胞菌属中的一个新物种。该新物种的拟议名称是甘蔗假单胞菌，类型菌株为MS586 ^T（登录号NRRL B-65441 =登录号LMG 30275）。