Multicenter evaluation of Xpert HIV-1 viral load assay for HIV quantification in China.,Journal of Medical Virology

当前位置： X-MOL 学术 › J. Med. Virol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Multicenter evaluation of Xpert HIV-1 viral load assay for HIV quantification in China.
Journal of Medical Virology ( IF 6.8 ) Pub Date : 2020-07-11 , DOI: 10.1002/jmv.26295
Lifeng Liu _{1,

2} , Renni Deng ₃ , Yun Lan ₄ , Li Li _{1,

2} , Mei Li ₃ , Bin Su _{1,

2} , Devasena Gnanashanmugam ₅ , Yi-Wei Tang ₅ , Hao Wu _{1,

2} , Jing Wang ₃ , Xiaoli Cai ₄ , Tong Zhang _{1,

2}

Affiliation

New approaches to increase HIV‐1 testing and HIV‐1 viral load (VL) monitoring are needed for people living with HIV (PLHIV) in China. The Xpert HIV‐1 VL assay was prequalified by the World Health Organization in 2017 but has not been evaluated in China. A multicenter evaluation was conducted to assess the accuracy of the Cepheid Xpert HIV‐1 VL assay compared to the Abbott RealTime HIV‐1 assay in China. Overall agreement was seen in 558 of 562 specimens (99.29%) with a κ value of 0.962. Pearson's coefficient between the two assays was 0.943. Analyzed by the Bland‐Altman method, the mean bias was −0.54 log₁₀ copies/mL, and 94.05% results fell within the 95% confidence limit of agreement (−1.248 to 0.168 log₁₀ copies/mL). The coefficient of variation of the Cepheid Xpert HIV‐1 VL assay ranged from 0.61% to 1.55%, as determined by testing eight positive plasma specimens with three different lots on different days. Due to its simplicity, random‐access, rapid turnaround time, and accuracy, the Xpert HIV‐1 VL assay can be used in local hospitals and clinics that bear the burden of identifying and treating HIV patients in China.

更新日期：2020-07-11

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11