A multi‐group screening method to detect residues of veterinary drugs in meat and environmental contaminants in wheat flour has been developed using liquid chromatography coupled to quadrupole‐Orbitrap high‐resolution mass spectrometry (LC‐HRMS). The procedure was tested for over 300 representative compounds (173 veterinary drugs, 122 pesticides and 9 mycotoxins) analysing in parallel negative and positive (spiked) samples according to European validation rules. The Screening Target Concentrations (STCs) were chosen conservatively with respect to the method purposes. Interpretation of results was based on retention time, mass accuracy of precursor and MS² spectral library. Evaluating the percentage of false negative results, 280 out of the 304 analytes were detectable at the STCs (false compliant rate ≤ 5%). In wheat flours, incurred levels of mycotoxins, deoxynivalenol and 3‐acetyldeoxynivalenol, higher than STCs, were frequently found, whereas in meat, the most detected veterinary drugs were antibiotics generally at negligible concentrations (<10 μg kg⁻¹). Finally, seven test materials from proficiency test schemes were successfully tested.

中文翻译：

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开发用于检测食品中真菌毒素、农药和兽药的多组筛查方法的实用方法。

使用液相色谱与四极杆轨道阱高分辨率质谱 (LC-HRMS) 联用，开发了一种多组筛查方法，用于检测肉类中的兽药残留和小麦粉中的环境污染物。根据欧洲验证规则，该程序对 300 多种代表性化合物（173 种兽药、122 种农药和 9 种真菌毒素）在平行的阴性和阳性（加标）样品中进行了测试。筛选目标浓度 (STC) 的选择相对于方法目的而言是保守的。结果的解释基于保留时间、母离子的质量准确度和 MS ²谱库。评估假阴性结果的百分比时，304 种分析物中的 280 种可在 STC 处检测到（假符合率 ≤ 5%）。在小麦面粉中，经常发现霉菌毒素、脱氧雪腐镰刀菌烯醇和 3-乙酰脱氧雪腐镰刀菌烯醇的含量高于 STC，而在肉类中，检测到最多的兽药是抗生素，通常浓度可以忽略不计（<10 μg kg ^-1）。最后，成功测试了能力验证计划中的七种测试材料。