Aberrant changes in the expression levels and structure of lipids may shape tumor microenvironment. In this study, we have performed mass spectrometry imaging and profiling analysis of 63 tissues of five types of cancer, namely, breast, colorectal, esophageal, lung, and gastric cancer, using in situ liquid extraction electrosonic spray ionization mass spectrometry imaging. Alteration of fatty‐acyl chain length of unsaturated phosphatidylcholines, phosphatidylinositols, and phosphatidylserines and of chain length of (un)saturated fatty acids are associated with different cancerous areas of five types of cancer. The ratios of the same fatty‐acyl carbon atom lipids with one double bond difference and the ratios of the same chain‐length fatty acids with one double bond difference exhibited significant differences among the cancerous areas of five types of cancer. Our data may reveal that there were different lipid metabolism networks among five types of cancer.

中文翻译：

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使用质谱成像原位探测癌变区域脂肪酰链长度的变化和脂质的去饱和度


脂质表达水平和结构的异常变化可能会塑造肿瘤微环境。在本研究中，我们采用原位液体提取电声喷雾电离质谱成像技术，对乳腺癌、结直肠癌、食管癌、肺癌和胃癌五种癌症的 63 个组织进行了质谱成像和谱分析。不饱和磷脂酰胆碱、磷脂酰肌醇和磷脂酰丝氨酸的脂肪酰基链长度的改变以及（不）饱和脂肪酸的链长度的改变与五种癌症的不同癌变区域相关。具有1个双键差异的相同脂肪酰基碳原子脂质的比例和具有1个双键差异的相同链长脂肪酸的比例在5种癌症的癌变区域中表现出显着差异。我们的数据可能揭示五种癌症之间存在不同的脂质代谢网络。