Aspergillus quadrilineatus endo-β-mannanase effectively degraded konjac glucomannan (66.09% w/v), copra meal (38.99% w/v) and locust bean galactomannan (20.94% w/v). High performance liquid chromatography (HPLC) analysis of KG hydrolysate indicated its mannooligosaccharides (MOS) content (656.38 mg/g) with high amounts of DP 5 oligosaccharide. Multi-scale characterization of mannan hydrolysate was done using FTIR and ¹³C NMR which revealed α and β form of galactose or glucose in MOS, respectively. CM and LBG hydrolysates (1 mg/mL) have shown cytotoxic effect and reduced cell viability of Caco-2 cells by 45% and 62%, respectively. MOS DP (1–4) derived from LBG supported better Lactobacilli biofilm formation as compared to KG hydrolysate containing high DP MOS (5–7). Lactobacilli effectively fermented MOS to generate acetate and propionate as main short chain fatty acids. Lactobacilli produced leucine, isoleucine and valine as branched chain amino acids when grown on LBG hydrolysate.

四线曲霉内切-β-甘露聚糖酶可有效降解魔芋葡甘露聚糖（66.09％w / v），椰干粕（38.99％w / v）和刺槐豆半乳甘露聚糖（20.94％w / v）。KG水解产物的高效液相色谱（HPLC）分析表明其甘露寡糖（MOS）含量（656.38 mg / g）和大量的DP 5寡糖。使用FTIR和¹³对甘露聚糖水解产物进行多尺度表征13 C NMR揭示了MOS中半乳糖或葡萄糖的α和β形式。CM和LBG水解产物（1 mg / mL）已显示出细胞毒性作用，并使Caco-2细胞的细胞活力分别降低了45％和62％。与含有高DP MOS的KG水解产物（5-7）相比，源自LBG的MOS DP（1-4）支持更好的乳杆菌生物膜形成。乳酸杆菌可有效发酵MOS，以产生乙酸盐和丙酸盐作为主要的短链脂肪酸。当在LBG水解产物上生长时，乳酸杆菌产生亮氨酸，异亮氨酸和缬氨酸作为支链氨基酸。