Multifunctional solvothermal carbon derived from alginate using 'water-in-deep eutectic solvents' for enhancing enzyme activity.,Chemical Communications

当前位置： X-MOL 学术 › Chem. Commun. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Multifunctional solvothermal carbon derived from alginate using 'water-in-deep eutectic solvents' for enhancing enzyme activity.
Chemical Communications ( IF 4.3 ) Pub Date : 2020-07-10 , DOI: 10.1039/d0cc03866k
Niketa Yadav ₁ , Manohara Halanur M , Meena Bisht , S K Nataraj , Pannuru Venkatesu , Dibyendu Mondal

Affiliation

Herein, the use of a “water-in-deep eutectic solvent (DES)” system has been shown as an alternative platform for the low temperature conversion of alginic acid (AA) to a multifunctional aliginate derived carbon (AAC) material with variable oxygen functionalities. Using the in-built oxygenated functionalities in AAC, cytochrome-c (Cyt-c) was immobilised on the surface of AACs and the peroxidase activity of the enzyme was studied. Remarkably, the enzymatic activity was enhanced up to 5.5-fold compared to the native protein without compromising the structural stability of Cyt-c. Altogether, the present study demonstrates a sustainable process for the preparation of AACs and shows their beneficial effect on the activity and stability of Cyt-c, and thus, their application as a protein-friendly material for biotechnological applications.

中文翻译：

使用“深层共晶溶剂”衍生自藻酸盐的多功能溶剂热碳，可增强酶的活性。

本文中，已显示出使用“深层共晶溶剂（DES）”系统作为将藻酸（AA）低温转化为具有可变氧的多官能链烷酸酯衍生碳（AAC）材料的替代平台。功能。使用AAC中内置的氧化功能，将细胞色素c（Cyt- c）固定在AAC的表面上，并研究了该酶的过氧化物酶活性。值得注意的是，与天然蛋白相比，酶促活性提高了5.5倍，而没有损害Cyt- c的结构稳定性。总而言之，本研究证明了制备AAC的可持续过程，并显示了它们对Cyt- c的活性和稳定性的有益作用。，因此，它们可作为生物技术应用中的蛋白质友好材料使用。

更新日期：2020-08-20

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11