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Study on Three Sarcocapnos Species as Potential Sources of Bioactive Compounds: Relation between Phenolic Content and Bioactivity by Multivariate Analysis.
Journal of Analytical Methods in Chemistry ( IF 2.3 ) Pub Date : 2020-07-11 , DOI: 10.1155/2020/8885169
María Del Pilar Fernández-Poyatos 1 , Gökhan Zengin 2 , Carlos Salazar-Mendías 3 , Antonio Ruiz-Medina 1 , Kouadio Ibrahime Sinan 2 , Eulogio J Llorent-Martínez 1
Affiliation  

In this work, we report the phenolic composition and bioactivity of the aerial parts of three species of Sarcocapnos (S. enneaphylla, S. pulcherrima, and S. saetabensis) to study their potential as sources of bioactive compounds to revalorize them and contribute to the conservation of these plant species. Samples were collected in different locations in the province of Jaén (southeast of Spain), and qualitative and quantitative analyses of phenolic compounds were performed by high-performance liquid chromatography with diode array and mass spectrometry detection. S. enneaphylla presented the highest concentration of phenolic compounds (58 mg/g DE). The most abundant compound in S. enneaphylla and S. saetabensis was rutin (35 mg/g DE and 11.7 mg/g DE, respectively), whereas isorhamnetin-O-rutinoside was dominant in S. pulcherrima (11.5 mg/g DE). Several assays were performed to evaluate the potential bioactivity of the three species of Sarcocapnos. These assays included antioxidant and radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating, and enzyme inhibitory activity (acetylcholinesterase, amylase, butyrylcholinesterase, glucosidase, and tyrosinase). In general, all methanolic extracts presented the highest phenolic and flavonoid contents, as well as the highest radical scavenging, antioxidant, and enzyme inhibitory properties. This relationship between phenolics and bioactivity was confirmed by multivariate analysis.

中文翻译:

作为生物活性化合物的潜在来源的三种Sarcocapnos物种的研究:酚含量与生物活性之间的关系通过多变量分析。

在这项工作中,我们报告的三个品种的地上部分的酚类成分和生物活性SarcocapnosS. enneaphyllaS.一品红S. saetabensis)研究其潜在的生物活性化合物的来源revalorize他们,并有助于这些植物物种的保护。在哈恩省(西班牙东南部)的不同地点收集了样品,并通过带有二极管阵列和质谱检测的高效液相色谱法对酚类化合物进行了定性和定量分析。芥菜链球菌的酚类化合物浓度最高(58 mg / g DE)。enne.phylla中最丰富的化合物SaetabensisS. saetabensis是芦丁(分别为35 mg / g DE和11.7 mg / g DE),而异鼠李素-O-芸香苷在S. pulcherrima中占主导地位(11.5 mg / g DE)。进行了几种测定,以评估这三种鲨鱼丝藻的潜在生物活性。。这些测定包括抗氧化剂和自由基清除剂(ABTS和DPPH),还原能力(CUPRAC和FRAP),磷钼和金属螯合以及酶抑制活性(乙酰胆碱酯酶,淀粉酶,丁酰胆碱酯酶,葡糖苷酶和酪氨酸酶)。通常,所有甲醇提取物均具有最高的酚和类黄酮含量,以及最高的自由基清除,抗氧化剂和酶抑制性能。多变量分析证实了酚类化合物与生物活性之间的这种关系。
更新日期:2020-07-13
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