Abstract

Purpose

Regarding the central role of insulin resistance in NAFLD, we explored whether insulin-like growth factor 1 (IGF1) and insulin-like growth factor-binding protein 3 (IGFBP3) gene variants were associated with NAFLD susceptibility.

Methods

IGF1 (rs6214) and IGFBP3 (rs3110697) gene variants were genotyped in 154 cases with biopsy-proven NAFLD and 156 controls using PCR-RFLP method.

Results

The IGF1 rs6214 “AA + AG” genotype compared with the “GG” genotype appeared to be a marker of decreased NAFLD susceptibility (p = .006; OR = 0.47, 95%CI = 0.28–0.80). Furthermore, the IGF1 rs6214 “A” allele was underrepresented in the cases than controls (p = .024; OR = 0.61, 95%CI = 0.40–0.94). However, we observed no significant difference in genotype or allele frequencies between the cases and controls for IGFBP3 gene.

Conclusions

To our knowledge, these findings suggest, for the first time, that the IGF1 rs6214 “A” allele and “AA + AG” genotype have protective effects for NAFLD susceptibility. Nonetheless, further studies are needed to validate our findings.

中文翻译：

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胰岛素样生长因子1（IGF1）基因rs6214的常见变异：非酒精性脂肪肝的潜在保护因素

摘要

目的

关于胰岛素抵抗在 NAFLD 中的核心作用，我们探讨了胰岛素样生长因子 1 ( IGF1 ) 和胰岛素样生长因子结合蛋白 3 ( IGFBP3 ) 基因变异是否与 NAFLD 易感性相关。

方法

使用 PCR-RFLP 方法对 154 例经活检证实的 NAFLD 和 156 例对照的IGF1 (rs6214) 和IGFBP3 (rs3110697) 基因变异进行了基因分型。

结果

与“GG”基因型相比，IGF1 rs6214“AA + AG”基因型似乎是 NAFLD 易感性降低的标志（p  = .006；OR = 0.47，95%CI = 0.28–0.80）。此外，IGF1 rs6214“A”等位基因在病例中的代表性低于对照组（p  = .024；OR = 0.61，95%CI = 0.40–0.94）。然而，我们观察到IGFBP3基因的病例和对照之间的基因型或等位基因频率没有显着差异。

结论

据我们所知，这些发现首次表明IGF1 rs6214“A”等位基因和“AA + AG”基因型对 NAFLD 易感性具有保护作用。尽管如此，还需要进一步的研究来验证我们的发现。