Abstract

In this study, the decolorization efficiency of seven microalgae isolates; Nostoc muscorum, Nostoc humifusum, Spirulina platensis, Anabaena oryzae, Wollea saccata, Oscillatoria sp. and Chlorella vulgaris was investigated for dye decolorization. The highest decolorization percentages of Brazilwood, Orange G, and Naphthol Green B dyes (99.5%, 99.5%, and 98.5%, respectively) were achieved by Chlorella vulgaris. However, the maximum efficiency for dye decolorization percentages of CV and malachite green dyes were exhibited by A. oryzae (97.4%) and W. saccata (93.3%). Ligninolytic enzymes activity assay was carried out for laccase and lignin peroxidase enzymes, which revealed a high efficiency of the C. vulgaris, A. oryzae and W. saccata to lignin containing compound degradation. The highest laccase production recorded by C. vulgaris with Brazilwood, Orange G, and Naphthol Green B dyes (665.0, 678.6, and 659.5 U/ml, respectively). Similarly, C. vulgaris gave a high lignin peroxidase enzyme production with the above three dyes respectively (306.00, 298.34, and 311.45 U/ml). In addition, A. oryzae and W. saccata showed the highest production of the laccase enzyme (634.6 and 577.45 U/ml, respectively) with CV and malachite green dyes. The degradation products have been characterized after decolorization and verified using FTIR analysis. The high decolorization percentages achieved by C. vulgaris, A. oryzae and W. saccata make them potential candidates for bioremediation and pre-processing to remove dyes from textile effluents.

摘要

在这项研究中，七个微藻分离物的脱色效率。发菜muscorum，发菜humifusum，螺旋藻，鱼腥藻菌，Wollea长毛盘，颤藻。和小球藻进行了研究用于染料脱色。小球藻实现了巴西木，橙G和萘酚绿B染料最高的脱色百分比（分别为99.5％，99.5％和98.5％）。然而，米曲霉（97.4％）和W. saccata表现出CV和孔雀绿染料的染料脱色率的最大效率。（93.3％）。木质素降解酶的活性测定法进行漆酶和木质素过氧化物酶的酶，它揭示了一个高效率小球藻，米曲霉和W.长毛盘到含木质素化合物的降解。用巴西木，橙色G和萘酚绿B染料记录的寻常梭菌漆酶产量最高（分别为665.0、678.6和659.5 U / ml）。类似地，寻常型衣原体用上述三种染料（306.00、298.34和311.45U / ml）分别产生高的木质素过氧化物酶酶。另外，米曲霉和萨卡塔酵母CV和孔雀绿染料显示出最高的漆酶产量（分别为634.6和577.45 U / ml）。脱色后对降解产物进行了表征，并使用FTIR分析进行了验证。寻常小球藻，米曲霉和沙门氏菌实现的高脱色率使其成为生物修复和预处理以从纺织品废水中去除染料的潜在候选者。