Abstract

This research aimed at implementing and validating a method for analysis of pesticide residues in crops. QuEChERS extraction method with PSA purification was used following analyzes by gas chromatography with tandem mass spectrometry in Selected Reaction Monitoring mode. A short run method was successfully developed for the determination of 41 pesticides, confirmed by two precursor-products for each analyte. The calibration curve for each analyte was linear at concentration range from 1 to 500 μg kg⁻¹ with correlation coefficients higher than 0.99, low limits of detection (0.03 − 10.22 μg kg⁻¹) and satisfactory precision. The developed method was used to investigate apples; mangos; strawberries; cucumbers and tomatoes from the Rio de Janeiro Food Distribution Center (CEASA).Most of the targeted pesticides (78%) were below detection limits. Apple and strawberry presented the highest pesticide contamination levels, many of which are not authorized by tthe Brazilian national regulatory agency (ANVISA).

摘要

这项研究旨在实施和验证一种用于分析农作物中农药残留的方法。在选定的反应监测模式下，通过气相色谱-串联质谱分析，使用PSA纯化的QuEChERS提取方法。已成功开发出一种用于测定41种农药的短期方法，每种分析物都有两种前体产物来确认。每种分析物的校准曲线在1至500μgkg ^-1的浓度范围内呈线性，相关系数高于0.99，检出限低（0.03 − 10.22μgkg ^-1）和令人满意的精度。所开发的方法用于研究苹果。芒果 草莓; 里约热内卢食品分配中心（CEASA）的黄瓜和西红柿。大多数目标农药（78％）均低于检出限。苹果和草莓的农药污染水平最高，其中许多未经巴西国家监管机构（ANVISA）授权。