Highly specialized grade of Schizothoracine fishe is primarily distributed in the drainage of Qinghai‐Tibet Plateau and its surrounding region, which has precious biological resources. However, overfishing and habitat destruction have seriously affected the germplasm resources and population genetic structure of HSG, so it is a significance to search for reliable genetic markers to protect its wild resources. In this work, 27 novel polymorphic microsatellite were firstly generated for the entire HSG based on the SLAF data of the grade and assess their polymorphism in HSG from 4 genera of 8 species. Among which W24, W41, W50 were existed only in 3–4 populations with low amplification efficiency, and loci of W3, W6, W12, W25, W28, and W32 were absent in 1–3 species, the rest of that 18 loci were successfully amplified in all specimens. The average of the number of alleles (NA) per locus was 46.04, ranging from 12 to 79, and the average observed and expected heterozygosities were estimated from 0.539 to 0.785 and 0.471 to 0.669 in 8 species, respectively. The average polymorphic information content (PIC) of each species was from 0.669 to 0.433. The range of average Shannon‐Weiner diversity index (H') were calculated from 0.914 to 1.717 in 8 species. To sum up, we developed 27 microsatellite loci with higher quality and stability, which could provide effective technical support for the study of germplasm resources of highly specialized grade of schizothoracine fishe.

中文翻译：

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基于SLAF-seq技术的裂殖壶菌鱼（鲤形目：鲤科）SSR标记的表征和开发

高度专业化的裂殖壶菌鱼主要分布在青藏高原及其周边地区的流域中，这具有宝贵的生物资源。然而，过度捕捞和栖息地破坏严重影响了HSG的种质资源和种群遗传结构，因此寻找可靠的遗传标记来保护其野生资源具有重要意义。在这项工作中，首先根据该等级的SLAF数据为整个HSG生成了27个新颖的多态微卫星，并评估了8种4属HSG中它们的多态性。其中W24，W41，W50仅存在于3-4个扩增效率较低的种群中，而1-3个物种中不存在W3，W6，W12，W25，W28和W32的基因座，其余18个基因座是在所有标本中均成功扩增。每个基因座的等位基因（NA）平均数为46.04，范围从12到79，估计的平均杂合度和预期杂合度在8个物种中分别为0.539至0.785和0.471至0.669。每个物种的平均多态信息含量（PIC）为0.669至0.433。计算了8种物种的平均Shannon-Weiner多样性指数（H'）的范围从0.914至1.717。综上所述，我们开发了质量和稳定性更高的27个微卫星基因座，可为研究高度专业级的裂殖壶菌鱼类的种质资源提供有效的技术支持。每个物种的平均多态信息含量（PIC）为0.669至0.433。计算了8种物种的平均Shannon-Weiner多样性指数（H'）的范围从0.914至1.717。综上所述，我们开发了质量和稳定性更高的27个微卫星基因座，可以为研究高度专业化的裂殖壶菌鱼种质资源提供有效的技术支持。每个物种的平均多态信息含量（PIC）为0.669至0.433。计算了8种物种的平均Shannon-Weiner多样性指数（H'）的范围从0.914至1.717。综上所述，我们开发了质量和稳定性更高的27个微卫星基因座，可以为研究高度专业化的裂殖壶菌鱼种质资源提供有效的技术支持。