Costus plant is considered as a medicinal plant especially in therapeutic intervention. The current study reports the composition, phytochemical, and antioxidant properties of red button ginger (Costus woodsonii) using sequential extraction. Solvents of different polarities (hexane, ethyl acetate, methanol, and water) were used to sequentially extract the C. woodsonii bract, at increasing polarities. Phytochemical screening showed positive presence of flavonoids (all types of extracts), terpenoids (only in methanolic extract), and tannins (except aqueous extract). Overall, methanolic extracts showed the significantly (p ≤ .05) highest ascorbic acid content (75.76 ± 1.47 mg/100 ml), total phenolic content (249.64 ± 2.83 mg GAE/100 g), DPPH free radical‐scavenging activity (78.76 ± 0.97%), ferric reducing antioxidant power (22.55 ± 0.96 mg TEAC/g), superoxide anion scavenging activity (46.60 ± 1.57%) and hydroxyl radical‐scavenging activity (50.35 ± 0.96%). In short, sequential extraction provides understanding of polarity specificity of C. woodsonii plant and result indicated high polarity solvent (methanol and water) recovered higher bioactive compounds.

中文翻译：

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顺序提取红扣姜（Costus woodsonii）：植物化学筛选和抗氧化活性

椰子属植物被认为是药用植物，特别是在治疗干预中。目前的研究报道了采用连续提取法得到的红扣姜（Costus woodsonii）的组成，植物化学和抗氧化特性。使用极性不同的溶剂（己烷，乙酸乙酯，甲醇和水）以递增的极性顺序提取伍德森梭菌片。植物化学筛选显示，类黄酮（所有类型的提取物），萜类化合物（仅在甲醇提取物中）和单宁（水提取物除外）呈阳性存在。总体来看，甲醇提取物表现出显著（p ≤.05）最高抗坏血酸含量（75.76±1.47 mg / 100 ml），总酚含量（249.64±2.83 mg GAE / 100 g），DPPH清除自由基活性（78.76±0.97％），三价铁还原抗氧化能力（ 22.55±0.96 mg TEAC / g），超氧阴离子清除活性（46.60±1.57％）和羟自由基清除活性（50.35±0.96％）。简而言之，顺序提取提供了对伍德森梭菌植物极性特异性的理解，结果表明高极性溶剂（甲醇和水）可回收较高的生物活性化合物。