The prevalence of periodontal disease poses a significant global health burden. Treatments for these diseases, primarily focused on removal and eradication of dental plaque biofilms, are challenging due to limited access to periodontal pockets where these oral pathogens reside. Herein, we report on the development and characterization of nitric oxide (NO)‐releasing carboxymethylcellulose (CMC) derivatives and evaluate their in vitro bactericidal efficacy against planktonic Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, two prominent periodontopathogens. Bactericidal exposure assays revealed that three of the synthesized NO‐releasing polymers were capable of reducing bacterial viability of both species by 99.9% in 2 hr at concentrations of 4 mg ml⁻¹ or lower, reflecting NO's potent and rapid bactericidal action. The NO‐releasing CMCs elicited minimal toxicity to human gingival fibroblasts at their bactericidal concentrations following 24‐hr exposure.

中文翻译：

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释放一氧化氮的羧甲基纤维素对牙周病原菌的抗菌活性

牙周病的流行给全球健康带来了沉重的负担。这些疾病的治疗，主要集中在去除和根除牙菌斑生物膜，由于进入这些口腔病原体所在的牙周袋的机会有限，因此具有挑战性。在此，我们报告了释放一氧化氮 (NO) 的羧甲基纤维素 (CMC) 衍生物的开发和表征，并评估了它们对浮游牙龈卟啉单胞菌和伴放线放线菌这两种主要牙周病原体的体外杀菌效果。^{杀菌暴露试验表明，在浓度为 4 mg ml -1}的情况下，三种合成的 NO 释放聚合物能够在 2 小时内将两种细菌的​​细菌活力降低 99.9%或更低，反映了 NO 的强效和快速杀菌作用。在 24 小时暴露后，释放 NO 的 CMC 在其杀菌浓度下对人牙龈成纤维细胞的毒性最小。