Recent studies have highlighted a potential role of genetic and epigenetic variation in the development of Alzheimer’s disease. Application of the CRISPR‐Cas genome‐editing platform has enabled investigation of the functional impact that Alzheimer’s disease‐associated gene mutations have on gene expression. Moreover, recent advances in the technology have led to the generation of CRISPR‐Cas–based tools that allow for high‐throughput interrogation of different risk variants to elucidate the interplay between genomic regulatory features, epigenetic modifications, and chromatin structure. In this review, we examine the various iterations of the CRISPR‐Cas system and their potential application for exploring the complex interactions and disruptions in gene regulatory circuits that contribute to Alzheimer’s disease.

中文翻译：

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应用基因编辑技术阐明阿尔茨海默病遗传和表观遗传变异的功能后果。


最近的研究强调了遗传和表观遗传变异在阿尔茨海默病发展中的潜在作用。 CRISPR-Cas基因组编辑平台的应用使得研究阿尔茨海默病相关基因突变对基因表达的功能影响成为可能。此外，该技术的最新进展催生了基于 CRISPR-Cas 的工具，这些工具允许对不同风险变异进行高通量询问，以阐明基因组调控特征、表观遗传修饰和染色质结构之间的相互作用。在这篇综述中，我们研究了 CRISPR-Cas 系统的各种迭代及其在探索导致阿尔茨海默病的基因调控回路中复杂的相互作用和破坏方面的潜在应用。