Abstract Parkinson's disease (PD) is one of the most common neurodegenerative diseases (NDD) and mainly affects the ageing population. A significant feature of PD pathogenesis is the progressive loss of dopaminergic neurons in the substantia nigra pars compacta part of the midbrain in affected persons. This neuronal loss occurs partly due to the increased generation of reactive oxygen species (ROS) in the mitochondrial and the depletion of adenosine triphosphate (ATP) in affected neurons. In this study, the methanolic extracts of Clivia miniata (CME) and Nerine humilis (NHE) belonging to the plant family Amaryllidaceae, were investigated for their neuroprotective potential in MPP+-induced neurotoxicity in SH-SY5Y neuroblastoma cells. Cell viability was determined using the 3-(4,5-dimethyithiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays and cell morphology was analysed using light microscopy. Furthermore, the effects of the extracts on apoptosis and ATP production were investigated using caspase 3/7 apoptosis kit and the Promega Mitochondrial ToxGlo ATP assay kit respectively. Additionally, the antioxidant contents of extracts were evaluated using routine laboratory procedures. The results show that pre-treatment of cells with the extracts at 2 and 4 μg/mL concentrations improved cell viability as well as cell morphology by inhibiting the toxicity induced by MPP+. The extracts also improved ATP levels in cells and attenuated the apoptosis induced by MPP+. Furthermore, antioxidant assays showed that both extracts had low antioxidant activity. Findings from this study indicate that CME and NHE may be promising as neuroprotective agents for PD and warrant further investigation to determine the bioactive components of the plants that may be responsible for the observed effects.

中文翻译：

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Clivia miniata 和 Nerine humilis（石蒜科）在 MPP+ 诱导的 SH-SY5Y 神经母细胞瘤细胞神经元毒性中的体外神经保护潜力

摘要 帕金森病（PD）是最常见的神经退行性疾病（NDD）之一，主要影响人口老龄化。PD 发病机制的一个显着特征是受影响人中脑的黑质致密部中的多巴胺能神经元逐渐丧失。这种神经元丢失的部分原因是线粒体中活性氧 (ROS) 的产生增加以及受影响神经元中三磷酸腺苷 (ATP) 的消耗。在这项研究中，研究了属于植物家族石蒜科的君子兰 (CME) 和 Nerine humilis (NHE) 的甲醇提取物在 MPP+ 诱导的 SH-SY5Y 神经母细胞瘤细胞神经毒性中的神经保护潜力。使用 3-(4,5-dimethythiazol-2-yl)-2 测定细胞活力，使用光学显微镜分析了 5-二苯基溴化四唑 (MTT) 测定和细胞形态。此外，分别使用 caspase 3/7 细胞凋亡试剂盒和 Promega Mitochondrial ToxGlo ATP 检测试剂盒研究提取物对细胞凋亡和 ATP 产生的影响。此外，使用常规实验室程序评估提取物的抗氧化剂含量。结果表明，用 2 μg/mL 和 4 μg/mL 浓度的提取物预处理细胞通过抑制 MPP+ 诱导的毒性提高了细胞活力和细胞形态。提取物还提高了细胞中的 ATP 水平，并减弱了 MPP+ 诱导的细胞凋亡。此外，抗氧化试验表明，两种提取物的抗氧化活性都很低。