Abstract The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. indica plants were collected, surface sterilized and cultured in Schenk and Hildebrandt (SH) media fortified with various PGRs and PAs in different concentrations and combinations. SH media fortified with benzylaminopurine (BAP) (2.0 mg/l)+put (0.5 mM) and in another experiment, BAP (2.0 mg/l)+spd (1 mM) produced best results with 41.00 and 39.2 axillary shoots/ nodal explant respectively. Following 4-6 weeks of incubation, explants propagated in SH media augmented with various combinations and concentrations of BAP and kinetin (kin) (0.5, 1, 1.5 and 2 mg/l), spm, spd and put (0.5 and 1 mM) generated white friable callus. SH media supplemented with BAP (2.0 mg/l)+spd (0.5 mM) displayed best response with an average number of 47.5 base callus derived shoots . Following 6 weeks of incubation, SH+ indole-3-acetic acid (IAA) (1.0 mg/l)+spd (0.5 mM) resulted maximum average number (7) of roots per shoot. The genetic homogeneity of the mother plant (MP), hardened plant (HP) and in vitro regenerated plants was ascertained employing random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. In this present study, in randomly selected A. indica plants, by using 6 ISSR and 5 RAPD primers, 63 scorable bands were derived, 59 of which were recorded as monomorphic with a degree of monomorphism of 90%. High-performance thin layer chromatography (HPTLC) revealed aristolochic acid (AA) content in in vitro grown A. indica roots and the roots from the mother plant (MP) as 6074.54 µg/g and 5891.14 µg/g respectively.

中文翻译：

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多胺引发马兜铃酸在体外克隆性fidel马兜铃中产生：基于ISSR和RAPD标记和HPTLC的研究

摘要 本研究旨在找出不同多胺（PA）的影响，即。精胺 (spm)、亚精胺 (spd) 和腐胺 (put) 以及植物生长调节剂 (PGR) 对马兜铃体外增殖、生根、愈伤组织和再生的影响。首先，将籼稻植物收集、表面消毒并在强化有不同浓度和组合的各种 PGR 和 PA 的 Schenk 和 Hildebrandt (SH) 培养基中培养。用苄氨基嘌呤 (BAP) (2.0 mg/l)+put (0.5 mM) 强化的 SH 培养基，在另一个实验中，BAP (2.0 mg/l)+ spd (1 mM) 产生了最好的结果，41.00 和 39.2 腋芽/节点外植体分别。培养 4-6 周后，在 SH 培养基中繁殖的外植体增加了各种组合和浓度的 BAP 和激动素 (kin) (0.5, 1, 1. 5 和 2 毫克/升）、spm、spd 和 put（0.5 和 1 mM）生成白色易碎愈伤组织。补充有 BAP (2.0 mg/l)+spd (0.5 mM) 的 SH 培养基显示出最佳响应，平均有 47.5 个碱基愈伤组织衍生的芽。孵育 6 周后，SH+ 吲哚-3-乙酸 (IAA) (1.0 mg/l) + spd (0.5 mM) 导致每个芽的平均根数最大 (7)。使用随机扩增多态性 DNA (RAPD) 和简单序列间重复 (ISSR) 标记确定母株 (MP)、硬化植物 (HP) 和体外再生植物的遗传同质性。在本研究中，在随机选择的籼稻植物中，通过使用 6 个 ISSR 和 5 个 RAPD 引物，获得了 63 个可评分条带，其中 59 个被记录为单态性，单态性程度为 90%。