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Subsequent inclusion/exclusion criteria to select the best species for an experiment performed on the ISS in a refurbished hardware
Life Sciences in Space Research ( IF 2.9 ) Pub Date : 2020-07-11 , DOI: 10.1016/j.lssr.2020.07.002
Giovanna Aronne 1 , Leone Ermes Romano 1 , Luigi Gennaro Izzo 1
Affiliation  

The interest in re-using flown hardware for new and different space biology experiments is increasing. To match the constraints of the flown hardware with the requirements of the new biological system, innovative methodological approaches are necessary. MULTITROP was a successful plant biology experiment that was performed on the ISS to investigate multiple-tropism interactions during the early stage of seedling growth. We used the hardware designed and flown for the IFOAM experiment in 2009. The main challenge was to implement seeds of a crop species in a growth chamber conceived for yeast culture and to grow the seedlings in microgravity condition but activating seed germination on ground before the launch. Our approach was to adapt the biological system to the hardware constraints and also to the experiment timing and the environmental factors expected during the prelaunch, launch and flight operations. We looked for an objective and repeatable method to effectively select the best suited species. Innovatively, we applied the method of inclusion/exclusion criteria to adapt a new biological system to a reused hardware. The list and the consecutive order of the specific inclusive/exclusive criteria turned out to be a valid support to guide the science team in objectively choosing the most suitable species for the experiment. Among the 50 initial food species, the carrot seeds resulted as the best in satisfying all technical requirements and post-flight data confirmed the expectations.



中文翻译:

随后的纳入/排除标准为在翻新硬件中的国际空间站上进行的实验选择最佳物种

在新的和不同的空间生物学实验中重新使用飞行硬件的兴趣正在增加。为了使飞行硬件的约束与新生物系统的要求相匹配,需要创新的方法论方法。MULTITROP 是在国际空间站上进行的一项成功的植物生物学实验,用于研究幼苗生长早期的多向性相互作用。我们在 2009 年使用了为 IFOAM 实验设计和飞行的硬件。主要挑战是在为酵母培养而设计的生长室中实施作物物种的种子,并在微重力条件下培育幼苗,但在发射前在地面上激活种子发芽. 我们的方法是使生物系统适应硬件限制以及在预发射、发射和飞行操作期间预期的实验时间和环境因素。我们寻找一种客观且可重复的方法来有效地选择最适合的物种。创新地,我们应用了纳入/排除标准的方法来使新的生物系统适应重复使用的硬件。具体包含/排除标准的列表和连续顺序被证明是指导科学团队客观选择最适合实验的物种的有效支持。在 50 种初始食品中,胡萝卜种子在满足所有技术要求方面表现最好,飞行后数据证实了预期。我们寻找一种客观且可重复的方法来有效地选择最适合的物种。创新地,我们应用了纳入/排除标准的方法来使新的生物系统适应重复使用的硬件。具体包含/排除标准的列表和连续顺序被证明是指导科学团队客观选择最适合实验的物种的有效支持。在 50 种初始食品中,胡萝卜种子在满足所有技术要求方面表现最好,飞行后数据证实了预期。我们寻找一种客观且可重复的方法来有效地选择最适合的物种。创新地,我们应用了纳入/排除标准的方法来使新的生物系统适应重复使用的硬件。具体包含/排除标准的列表和连续顺序被证明是指导科学团队客观选择最适合实验的物种的有效支持。在 50 种初始食品中,胡萝卜种子在满足所有技术要求方面表现最好,飞行后数据证实了预期。具体包含/排除标准的列表和连续顺序被证明是指导科学团队客观选择最适合实验的物种的有效支持。在 50 种初始食品中,胡萝卜种子在满足所有技术要求方面表现最好,飞行后数据证实了预期。具体包含/排除标准的列表和连续顺序被证明是指导科学团队客观选择最适合实验的物种的有效支持。在 50 种初始食品中,胡萝卜种子在满足所有技术要求方面表现最好,飞行后数据证实了预期。

更新日期:2020-07-11
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