Single-molecule fluorescence microscopy techniques have enabled the lifecycle of individual RNA transcripts to be quantitatively measured in living cells. The application of these approaches to monitor mRNA degradation, however, has presented a challenge to unequivocally detect these events due to the inherent loss-of-signal resulting from decay of a transcript. Here, we highlight the recent technological developments that have enabled the spatial and temporal dynamics of mRNA degradation of individual transcripts to be visualized within living cells.

中文翻译：

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从活细胞中的单分子成像深入了解 mRNA 降解。

单分子荧光显微镜技术使单个 RNA 转录本的生命周期能够在活细胞中进行定量测量。然而，由于转录本衰减导致固有的信号丢失，应用这些方法来监测 mRNA 降解对明确检测这些事件提出了挑战。在这里，我们强调了最近的技术发展，这些发展使单个转录本的 mRNA 降解的空间和时间动态能够在活细胞内可视化。