Plants of Potamogeton crispus, a submersed macrophyte, were cultivated in the water free of algae and containing different concentrations of inorganic nitrogen (0.2 and 20 mg N L⁻¹) and phosphorus (0.02 and 2.0 mg P L⁻¹). The changes in plant growth and turion formation and the carbohydrate contents in plants and turions were investigated, and the endogenous abscisic acid (ABA) levels and the expressions of ABA biosynthetic genes in the stem apices analysed. The purpose of this study was to clarify the effects of water N and P levels on the plant growth and propagation capacity of P. crispus and to reveal whether ABA metabolism is involved in the turion formation of the plant under different ambient nutritional conditions. Finding the biomass of plants was not affected by the water N and P concentrations, except for those grown in water with low N and high P that had higher biomass accumulation and carbohydrate contents. Higher P promoted plant N uptake from the water, but higher N inhibited plant P uptake from the water with high P. High water N significantly delayed the turion initiation of P. crispus. Plants formed more apical turions with a greater size and more carbohydrate reserves when grown in water containing low N and low P, which leads to higher propagation of the plant population. The combination of high N and high P in water inhibited the formation and growth of turions, resulting in significant attenuation of the propagation capacity. High N combinated with low P or high P combinated with low N promoted the formation of more axillary turions and total turions but inhibited the turion growth. ABA levels and the expressions of ABA biosynthetic genes in stem apices at the turion initiation were changed by water N and P levels. The elevated ABA levels were consistent with the increased total turion number per plant when grown in the water with low N and/ or low P. The up-regulation of ZEP (zeaxanthin epoxidase) or NCED6 (9-cis-epoxycarotenoid dioxygenase6) may account for the ABA accumulation in the stem apices. The involvement of ABA metabolism in the turion formation relies on varying gene expression patterns when the plant grows in water containing different N and P concentrations.

在没有藻类的水中种植了潜入的大型植物Potamogeton crispus的植物，该水中含有不同浓度的无机氮（0.2和20 mg NL ^-1）和磷（0.02和2.0 mg PL ^-1）。研究了植物生长和扭节形成的变化，以及植物和扭节中碳水化合物的含量，分析了茎尖内源脱落酸（ABA）的含量和ABA生物合成基因的表达。本研究的目的是阐明水氮和磷水平对植物生长和磷传播能力的影响。脆皮并揭示在不同的环境营养条件下，ABA代谢是否与植物的扭体形成有关。寻找植物的生物量不受水氮和磷浓度的影响，除了那些生长在低氮和高磷的水中的植物具有较高的生物量积累和碳水化合物含量。较高的P促进植物从水中吸收N，但是较高的N抑制高P的植物对植物P的吸收。较高的N会显着延迟P的变构起始。脆皮。当植物在低氮和低磷的水中生长时，它们会形成更大的顶端，并具有更大的大小和更多的碳水化合物储备，从而导致植物种群的更高繁殖。水中高氮和高磷的结合抑制了线虫的形成和生长，导致繁殖能力大大降低。高氮与低磷结合或高磷与低氮结合促进了更多的腋毛变种和总变种的形成，但抑制了变种的生长。氮素和磷素的水平改变了鞭毛开始时茎尖中ABA的含量和ABA生物合成基因的表达。升高的ABA水平符合在水中具有低N和/或低P上生长时的增加的每株植物的总的Turion数上调的ZEP（玉米黄质环氧酶或NCED6（9-顺式-环氧类胡萝卜素双加氧酶6）可能是造成茎尖ABA积累的原因。当植物在含有不同N和P浓度的水中生长时，ABA代谢参与扭体形成依赖于不同的基因表达模式。