Abstract In this study, the mixed strains, including Lactobacillus plantarum (STBL1), Saccharomyces cerevisiae (STBS1), and Bacillus safensis (SQVG18) were applied to the solid-state fermentation of commercial feed for Penaeus vannamei. Firstly, the optimum combination of strain inoculum, optimum fermentation conditions, and nutritional quality of the fermented feeds were investigated. It was noted that the acid-soluble protein and the content of amino acids (lysine, methionine, etc.) were remarkably improved in the feeds by solid-state fermentation with the optimum combination of STBL1: STBS1: SQVG18 = 1: 3: 3. Secondly, a six-week feeding experiment was performed using one control diet without probiotics (D0: 0 CFU g−1) and three fermented diets containing different concentrations of the optimum mixed-strain combination (D1: 107 CFU g−1; D2: 108 CFU g−1; D3: 109 CFU g−1). Shrimps fed D2, and D3 groups exhibited significantly better growth performance and activities of antioxidant enzymes and digestive enzymes than shrimp of other groups (P

中文翻译：

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混合菌种发酵饲料的研究及其对南美白对虾存活、生长、消化酶活性和肠道菌群的影响

摘要 本研究将植物乳杆菌（STBL1）、酿酒酵母（STBS1）和安全芽孢杆菌（SQVG18）等混合菌株应用于南美白对虾商品饲料的固态发酵。首先对菌种的最佳组合、最佳发酵条件和发酵饲料的营养品质进行了考察。注意到采用STBL1：STBS1：SQVG18=1：3：3的最佳组合固态发酵，饲料中酸溶性蛋白质和氨基酸（赖氨酸、蛋氨酸等）含量显着提高. 其次，使用一种不含益生菌的对照日粮（D0：0 CFU g-1）和含有不同浓度最佳混合菌株组合的三种发酵日粮（D1：107 CFU g-1；D2 ：108 CFU g−1；D3：109 CFU g-1)。饲喂D2、D3组的虾表现出明显优于其他组虾的生长性能和抗氧化酶和消化酶的活性（P