Zymomonas mobilis is an ethanologenic microbe that has a demonstrated potential for use in lignocellulosic biorefineries for bioethanol production. Z. mobilis exhibits a number of desirable characteristics for use as an ethanologenic microbe, with capabilities for metabolic engineering and bioprocess modification. Many advanced genetic tools, including mutation techniques, screening methods and genome editing have been successively performed to improve various Z. mobilis strains as potential consolidated ethanologenic microbes. Many bioprocess strategies have also been applied to this organism for bioethanol production. Z. mobilis biofilm reactors have been modified with various benefits, including high bacterial populations, less fermentation times, high productivity, high cell stability, resistance to the high concentration of substrates and toxicity, and higher product recovery. We suggest that Z. mobilis biofilm reactors could be used in bioethanol production using lignocellulosic substrates under batch, continuous and repeated batch processes.

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在乙醇生产中使用运动发酵单胞菌进行生物工艺优化的前景和新方向

运动发酵单胞菌是一种产乙醇微生物，在木质纤维素生物精炼厂用于生物乙醇生产中具有已证明的潜力。运动发酵单胞菌表现出许多用作产乙醇微生物的理想特性，具有代谢工程和生物过程改造的能力。许多先进的遗传工具，包括突变技术、筛选方法和基因组编辑，已相继进行，以将各种运动发酵单胞菌菌株改进为潜在的整合产乙醇微生物。许多生物过程策略也已应用于该生物体以生产生物乙醇。运动发酵单胞菌生物膜反应器经过改进，具有多种优势，包括高细菌种群、更少的发酵时间、高生产力、高细胞稳定性、耐高浓度底物和毒性，产品回收率更高。我们建议运动发酵单胞菌生物膜反应器可用于在分批、连续和重复分批过程中使用木质纤维素基质生产生物乙醇。