Endophytic fungi are not only ubiquitous in plants, but also play an important role in the balancing of plant microecosystems. Numerous studies have shown that plant species and environment have a significant influence on the richness of endophytic fungi, and endophytic fungi often provide different forms of fitness benefits to their associated host plants. The present work aimed to isolate and identify endophytic fungi harboured in the tissues of the native medicinal plant Cornus officinalis and screen beneficial fungi to inhibit cornel pathogens. A total of 208 strains were recovered from 720 tissue segments. The overall colonisation and isolation rates of endophytic fungi were 33.89% and 28.89%, with triennial twigs (53.33%, 55%) and fruits (6.67%, 3.33%) having the highest and lowest rates, respectively. On the basis of morphological characteristics and internal transcribed spacer sequence analysis, 183 isolates were classified into 13 genera. Amongst them, Alternaria, Botryosphaeria and Talaromyces were dominant communities, and their relative abundances were 31.25%, 26.92% and 10.10%, respectively. A detailed calculation of the Shannon diversity (H′ = 1.65) and Margalef’s richness indices (Dmg = 2.30) revealed that the overall biodiversity of fungal endophytes in C. officinalis was relatively high, with the stems harbouring the highest diversity. The antagonism assay of 75 representative endophytes on the four main fungal pathogens of C. officinalis indicated that nine strains with antibiosis and eight strains with inhibition rate of more than 50% were obtained by the dual culture. To the best of our knowledge, this is the first study that investigated the distribution and antifungal activity of endophytic fungi from the medicinal plant C. officinalis.

中文翻译：

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药用山茱Corn内生真菌的生物多样性和抗真菌潜力

内生真菌不仅在植物中普遍存在，而且在植物微生态系统的平衡中也起着重要作用。大量研究表明，植物种类和环境对内生真菌的丰富性具有重大影响，而内生真菌通常为其相关的寄主植物提供不同形式的健身益处。本工作旨在分离和鉴定天然药用植物山茱off的组织中所含的内生真菌并筛选有益真菌以抑制山茱el病原体。从720个组织片段中总共回收了208个菌株。内生真菌的总定殖率和分离率分别为33.89％和28.89％，其中三年生嫩枝（53.33％，55％）和水果（6.67％，3.33％）的发生率最高和最低。根据形态特征和内部转录间隔区序列分析，将183个分离株分为13属。其中有链格孢属，葡萄孢菌和毛囊菌是优势社区，其相对丰度分别为31.25％，26.92％和10.10％。香农多样性（H'= 1.65）和丰富度的丰富度指数（DMG = 2.30）的详细计算表明，在内生真菌的生物多样性整体C.巴戟较高，与茎携带最高的多样性。对C. officinalis的四种主要真菌病原体进行75种代表性内生菌的拮抗分析，结果表明，通过双重培养获得了9种具有抗菌作用的菌株和8种抑制率超过50％的菌株。据我们所知，这是首次研究药用植物C. officinalis的内生真菌的分布和抗真菌活性的研究。。