An analytical method for the simultaneous quantitation of ten trichothecenes of type A (HT-2 toxin, T-2 toxin, diacetoxyscirpenol, and neosolaniol) and type B (3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, deoxynivalenol, deoxynivalenol-3-glucoside, nivalenol, and fusarenon-X) in feed has been developed using liquid chromatography with tandem mass spectrometry. Mycotoxins extracted twice from samples using aqueous acetonitrile were purified using a multifunctional clean-up column, followed by a phospholipid removal column. Trichothecenes were analysed using liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry. The extraction efficiency of the mycotoxins and the repeatability of some were improved by repeated extractions. Ionization enhancement (signal enhancement) of some mycotoxins was improved by using the phospholipid removal column at the clean-up step. Spike and recovery tests of trichothecenes were conducted on maize, barley, soybean meal, rapeseed meal, and formula feeds (for starting broiler chicks, suckling pigs, and beef cattle). The mean recovery values were 70.6–119% with relative standard deviations < 17%. The limit of quantification and the limit of detection of our method were 20 and 6 μg/kg, respectively, for 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol; 10 and 3 μg/kg, respectively, for T-2 toxin, deoxynivalenol, and fusarenon-X; and 5 and 2 μg/kg, respectively, for nivalenol and the remaining mycotoxins.

同时定量分析十种A型（HT-2毒素，T-2毒素，二乙酰氧基scirpenol和新Solaniol）和B型（3-乙酰基脱氧雪茄烯醇，15-乙酰基脱氧雪茄烯醇，脱氧雪茄烯醇，脱氧雪茄烯醇-3-葡糖苷，雪茄烯醇）的三色菊酯的分析方法，和饲料中的fusarenon-X）已通过液相色谱和串联质谱法开发。使用多功能净化柱，然后使用磷脂去除柱，对使用乙腈水溶液从样品中提取两次的霉菌毒素进行纯化。使用液相色谱大气压化学电离串联质谱法分析了单端孢菌。通过反复提取，可提高霉菌毒素的提取效率和某些真菌的重复性。在净化步骤使用磷脂去除柱可改善某些真菌毒素的电离增强（信号增强）。对玉米，大麦，大豆粉，菜籽粉和配方饲料（用于起始肉鸡，乳猪和肉牛）进行了毛霉菌病的加标和恢复测试。平均回收率为70.6–119％，相对标准偏差<17％。3-乙酰基脱氧雪茄烯醇和15-乙酰基脱氧雪茄烯醇的定量限和检测限分别为20和6μg/ kg；对于T-2毒素，脱氧雪腐烯酚和fusa​​renon-X，分别为10和3μg/ kg；奈芬烯醇和其余霉菌毒素分别为5和2μg/ kg。大豆粉，菜籽粉和配方饲料（用于肉鸡雏鸡，乳猪和肉牛）。平均回收率为70.6–119％，相对标准偏差<17％。3-乙酰基脱氧雪茄烯醇和15-乙酰基脱氧雪茄烯醇的定量限和检测限分别为20和6μg/ kg；对于T-2毒素，脱氧雪腐烯酚和fusa​​renon-X，分别为10和3μg/ kg；奈芬烯醇和其余霉菌毒素分别为5和2μg/ kg。大豆粉，菜籽粉和配方饲料（用于肉鸡雏鸡，乳猪和肉牛）。平均回收率为70.6–119％，相对标准偏差<17％。3-乙酰基脱氧雪茄烯醇和15-乙酰基脱氧雪茄烯醇的定量限和检测限分别为20和6μg/ kg；对于T-2毒素，脱氧雪腐烯酚和fusa​​renon-X，分别为10和3μg/ kg；奈芬烯醇和其余霉菌毒素分别为5和2μg/ kg。对于T-2毒素，脱氧雪腐烯酚和fusa​​renon-X，分别为10和3μg/ kg；奈芬烯醇和其余霉菌毒素分别为5和2μg/ kg。对于T-2毒素，脱氧雪腐烯酚和fusa​​renon-X，分别为10和3μg/ kg；奈芬烯醇和其余霉菌毒素分别为5和2μg/ kg。