A reusable optofluidic point-of-care testing platform (OPOCT) was successfully constructed through integrating evanescent wave fluorescence technology into an all-fiber-based optofluidic system. The compact design of the OPOCT allows it to be portable and suitable for on-site sensitive determination of biomarkers in serum without complicated and costly procedures. The sensitivity of 90.9 pM for antibody determination is observed thanks to the high transmission efficiency of excitation light and fluorescence in the OPOCT. The affinity constant between cholylglycine (CG) and anti-CG antibody was quantified using this platform based on the proposed theory. Using the lyophilized fluorescence-labeled specific antibody and reusable fiber optic biosensor, the OPOCT is applied to the one-step sensitive determination of CG in serum, which eliminates the dearth associated with liquid reagent handling, disposable biosensors, and user intervention. A limit of detection of 0.025 μg/mL for CG is obtained, which is far more than adequate for meeting diagnostic requirements. The matrix effect of serum samples on the evanescent wave–based optofluidic biosensor can be effectively reduced by simple dilution of serum samples. The performance of the OPOCT also compared favorably with that of a commercial turbidimetric inhibition immunoassay through analyzing multiple serum samples. This platform is ready to expand to measure any other biomarker by using its specific antibody. The simplicity, sensitivity, cost-effectiveness, and robustness of the OPOCT enable the early diagnosis of disease and making a timely clinical decision. Graphical abstract .

中文翻译：

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可重复使用的光流控床旁检测平台，带有冻干特异性抗体，用于血清中胆酰甘氨酸的荧光检测

通过将倏逝波荧光技术集成到全光纤光流控系统中，成功构建了可重复使用的光流控床旁检测平台（OPOCT）。OPOCT 的紧凑设计使其便于携带，适用于现场灵敏测定血清中的生物标志物，无需复杂且昂贵的程序。由于 OPOCT 中激发光和荧光的高传输效率，观察到抗体测定的灵敏度为 90.9 pM。胆酰甘氨酸 (CG) 和抗 CG 抗体之间的亲和力常数使用该平台基于所提出的理论进行量化。使用冻干荧光标记特异性抗体和可重复使用的光纤生物传感器，OPOCT应用于血清中CG的一步灵敏测定，这消除了与液体试剂处理、一次性生物传感器和用户干预相关的缺乏。CG 的检测限为 0.025 μg/mL，远远满足诊断要求。血清样品对基于倏逝波的光流体生物传感器的基质效应可以通过简单的血清样品稀释来有效降低。通过分析多个血清样品，OPOCT 的性能也优于商业比浊抑制免疫测定的性能。该平台已准备好扩展以使用其特定抗体测量任何其他生物标志物。OPOCT 的简单性、敏感性、成本效益和稳健性使疾病的早期诊断和及时做出临床决策成为可能。图形概要 。