Vibrio alginolyticus is posting an increasing threat to survival of grouper. Classical complement cascade can trigger initiation of immunity, while complement 9 (C9) is a major complement molecule involved in final step of membrane attack complex (MAC) formation. In this study, full-length EcC9 contained an ORF sequence of 1779 bp, encoding a polypeptide of 592 amino acids. A high-level expression of EcC9 mRNA was observed in liver. Following vibrio challenge, increased expression levels of EcC1q, EcBf/C2, EcC4, EcC6, EcC7 and EcC9 mRNA were detected in liver and kidney. These results implied that elevated expression level of classical complement pathway (CCP) and terminal complement components (TCCs) may assess toxicological effect of V. alginolyticus.

溶藻弧菌对石斑鱼生存的威胁越来越大。经典的补体级联反应可以触发免疫的启动，而补体9（C9）是参与膜攻击复合物（MAC）形成的最后一步的主要补体分子。在这项研究中，全长EcC9包含1779 bp的ORF序列，编码592个氨基酸的多肽。在肝脏中观察到EcC9 mRNA的高水平表达。弧菌攻击后，在肝脏和肾脏中检测到EcC1q，EcBf / C2，EcC4，EcC6，EcC7和EcC9 mRNA表达水平升高。这些结果暗示经典补体途径（CCP）和末端补体成分（TCC）的表达水平升高可以评估溶藻弧菌的毒理学作用。