Polysaccharide is the main active component of Dendrobium huoshanense, and it is also an important indicator to evaluate its quality. In this study, the polysaccharides and invertase components in the stems and leaves of D. huoshanense were studied to provide a theoretical basis for the full exploitation and utilization of its resources. Taking the D. huoshanense grown in the wild, indoors and outdoors as a test material and choosing stems and roots of D. huoshanense for 1 year, 2 years and 3 years of growth, the polysaccharide content of different samples was determined, and the relative expression activity of sucrose metabolism-related genes in different samples was detected by qRT-PCR. Western blot was used to detect the changes of sucrose metabolism-related proteins in different samples. The results showed that the content of polysaccharide in the wild D. huoshanense was the highest, the indoor growth was the second, the growth in the outdoor was the lowest, and the content of polysaccharide in the stem was significantly higher than that in the root. The expression of D. huoshanense neutral/alkaline invertase (DhNI), D. huoshanense sucrose synthase (DHSS) and D. huoshanense sucrose phosphate synthase (DHSPS) was the highest in wild D. huoshanense, followed by indoor growth, and the expression in stem was much higher than in root. Western blot results also indicated that DhNI, DHSS and DHSPS protein expression was highest in wild, followed by indoors. The stem is much higher than the root and is grown for 2 years in the same growing environment for more than 1 year and 3 years. The above results show that the nutritional value of D. huoshanense is related to the decomposition of sucrose and the accumulation of polysaccharides in plants. The experimental results provide a theoretical basis for better exploitation and utilization of D. huoshanense resources.

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不同处理对霍山石斛多糖含量相关基因的影响

多糖是霍山石斛的主要活性成分，也是评价其品质的重要指标。本研究对火山木茎叶中的多糖和转化酶成分进行了研究，为其资源的充分开发利用提供了理论依据。以野外、室内外生长的霍山毛蕊花为试验材料，选取生长1年、2年、3年的霍山毛蕊花茎、根，测定不同样品的多糖含量，并测定其相对含量。 qRT-PCR检测不同样品中蔗糖代谢相关基因的表达活性。Western blot检测不同样品中蔗糖代谢相关蛋白的变化。结果表明，野生霍山木多糖含量最高，室内生长次之，室外生长最低，茎中多糖含量显着高于根中。 . 火山果中性/碱性转化酶（DhNI）、火山果蔗糖合酶（DHSS）和火山果蔗糖磷酸合酶（DHSPS）的表达在野生霍山果中最高，其次是室内生长，在茎远高于根。Western印迹结果还表明，DhNI、DHSS和DHSPS蛋白在野外的表达量最高，其次是室内。茎比根高得多，在相同的生长环境下生长2年1年以上，3年以上。以上结果表明，霍山石斛的营养价值与植物体内蔗糖的分解和多糖的积累有关。试验结果为更好地开发利用霍山毛竹资源提供了理论依据。