The present study embarked on investigating the photo-fermentative bio-H₂ production kinetics and statistical optimization of operational parameters using immobilized inoculum from dark fermented palm oil mill effluent (DPOME). The statistical optimized values of initial pH, inoculum-substrate percentage (ISP), and light intensity were achieved by implicating the central composite design (CCD) under response surface methodology (RSM). The kinetic evaluation was conducted in terms of H₂-yield with ISP ranged from 10 to 30%. The highest H₂ production potential (P) and rate (R_max) of 30.59 ml.H₂/g-COD_removed and 0.514 ml h⁻¹ respectively were achieved with reported lag phase (ρ) value of 32 h by using 20% of ISP. However, an observed R_max of 0.746 with a ρ value of 39 h was attained at ISP of 15%. The quadratic model developed by using CCD was observed to display the significance value of < 00.5 of probability error. Additionally, observed values of correlation coefficient (R²), adequate precision (AP), and coefficient of variance (CV) for acquired H₂-yield were 0.9219, 12.076, and 11.24 ml.H₂/g-COD_removed, respectively. Under optimal conditions values (initial pH, 6.0; ISP, 20%; and light intensity, 350% W/m²), H₂ yield of 147 ml.H₂/g-COD_removed was achieved from DPOME, while reducing the COD value by 78 percentage. This statistical model is suggested to possess the regression, interaction, and quadratic terms as significant, so it can be deemed acceptable in terms of its achievable reproducibility.

本研究着手研究光发酵生物H _2的生产动力学，并使用来自黑暗发酵棕榈油厂出水（DPOME）的固定接种物对操作参数进行统计学优化。初始pH，接种底物百分比（ISP）和光强度的统计优化值是通过在响应面方法（RSM）下牵涉中央复合设计（CCD）来实现的。根据H ₂产率进行动力学评估，ISP范围为10％至30％。H ₂的最高生产潜力（P）和速率（R _max）为30.59 ml.H ₂ / g-COD_去除量为0.514 ml h ^-1通过使用20％的ISP，分别获得了报告的32 h滞后相位（ρ）值。但是，在15％的ISP下，观察到的R _max为0.746，ρ值为39 h。观察到使用CCD开发的二次模型显示出概率误差的显着性值<00.5。另外，相关系数（的观测值- [R ²为获取2H），足够的精度（AP），和方差（CV）的系数₂ -yield为0.9219，12.076，11.24和ml.H ₂ /克-COD_去除，分别。在最佳条件下的值（初始pH值为6.0； ISP为20％；光强度为350％W / m ²），H从DPOME中_除去2的147毫升H ₂ / g-COD ，同时将COD值降低了78％。建议该统计模型具有显着的回归，交互作用和二次项，因此就其可实现的可重复性而言，可以认为它是可接受的。