The air in public facilities contains microorganisms which may cause a wide variety of clinical syndromes in humans. This is particularly important in primary healthcare units, where patients with reduced immunity and staff are directly exposed to microbial pathogens. Air samples were collected from dispensing areas (customer areas) of five pharmacies using MAS-100 air sampler. The concentration of particular groups of microorganisms was determined with the use of selective media. The taxonomic analysis of mannitol-positive bacteria was conducted using the BIOLOG ® method, based on metabolic characteristics of analyzed strains. The isolated molds were identified based on their macroscopic and microscopic features according to available keys. The antimicrobial susceptibility of identified staphylococci was tested using the disk diffusion method in accordance with The European Committee on Antimicrobial Susceptibility Testing recommendations. The highest average concentrations of heterotrophic bacteria and staphylococci were recorded in two hospital pharmacies. The highest average concentrations of molds were recorded in the pharmacies located in shopping centers. Taxonomic analysis indicated that Cladosporium and Penicillium spp. predominated among the isolated molds while Staphylococcus haemolyticus and Staphylococcus aureus , among mannitol-positive bacteria. Antibiograms showed that the majority of Staphylococcus strains (68.2%) were resistant to erythromycin. There was a statistically significant correlation between the concentration of investigated bacteria and the season of the year as well as the size and location of the pharmacies. The results demonstrate that there is a range of factors affecting air quality in public spaces and emphasize the fact that pharmacies can be a source of potentially pathogenic, drug-resistant bacteria.

中文翻译：

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药房的微生物空气质量和葡萄球菌属的抗生素耐药性概况

公共设施中的空气中含有微生物，可能会导致人类出现多种临床症状。这在初级医疗保健单位尤为重要，因为在那里免疫力低下的患者和工作人员会直接接触微生物病原体。使用 MAS-100 空气采样器从五家药店的配药区（客户区）收集空气样本。使用选择性培养基测定特定微生物群的浓度。基于分析菌株的代谢特征，使用 BIOLOG ® 方法对甘露醇阳性细菌进行分类分析。根据可用的密钥，根据它们的宏观和微观特征来识别孤立的模具。根据欧洲抗菌药敏试验委员会的建议，使用圆盘扩散法测试已鉴定葡萄球菌的抗菌药敏性。两家医院药房记录了最高平均浓度的异养细菌和葡萄球菌。位于购物中心的药房记录的霉菌平均浓度最高。分类学分析表明 Cladosporium 和 Penicillium spp。在分离出的霉菌中占优势，而在甘露醇阳性菌中，溶血葡萄球菌和金黄色葡萄球菌占优势。抗菌谱显示大多数葡萄球菌菌株 (68.2%) 对红霉素有抗药性。研究的细菌浓度与一年中的季节以及药房的大小和位置之间存在统计学上显着的相关性。结果表明，有一系列因素会影响公共场所的空气质量，并强调了药房可能是潜在致病性耐药细菌的来源这一事实。