当前位置: X-MOL 学术RSC Adv. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Rapid characterization of the chemical constituents of Sanhua decoction by UHPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry
RSC Advances ( IF 3.9 ) Pub Date : 2020-7-10 , DOI: 10.1039/d0ra02264k
Yanhui Zhao 1 , Miao Wang 2 , Lin Sun 1 , Xue Jiang 1 , Min Zhao 1 , Chunjie Zhao 1
Affiliation  

Sanhua decoction, a famous Chinese herbal formula has been widely used for the treatment of stroke. In our study, a rapid, swift and straightforward analytical method with the help of UHPLC-FT-ICR-MS/MS was successfully developed for the first time to separate and identify the chemical constituents of Sanhua decoction. Chromatography was performed on a Universal XB C18 column (150 mm × 2.1 mm, 1.8 μm) using a mobile phase containing 0.1% formic acid–water (A) and acetonitrile (B). A total of 137 compounds in Sanhua decoction were identified or tentatively characterized. The findings revealed the fact that Sanhua decoction mainly contains flavonoids (in Aurantii fructus immaturus and Rheum palmatum L.), anthraquinones (in Rheum palmatum L.), coumarins (in Notopterygii Rhizoma Et Radix), phenylpropanoid glycosides, alkaloids and lignans (in Magnoliae Officmalis Cortex), which made up the key ingredients existing in Sanhua decoction. This study is hoped to be meaningful for the characterization of components in other traditional Chinese medicines, and lay the foundation for research on the pharmacology of Sanhua decoction.

中文翻译:

超高效液相色谱-傅里叶变换离子回旋共振质谱快速表征三花汤化学成分

三花汤是一种著名的中药方剂,已被广泛用于治疗中风。在我们的研究中,首次成功开发了一种借助 UHPLC-FT-ICR-MS/MS 快速、快速、直接的分析方法,用于分离鉴定三花汤的化学成分。使用含有 0.1% 甲酸-水 (A) 和乙腈 (B) 的流动相,在 Universal XB C 18色谱柱 (150 mm × 2.1 mm, 1.8 μm) 上进行色谱分析。共鉴定或初步鉴定了三花汤中的 137 种化合物。研究结果表明,三花汤主要含有黄酮类化合物(在枳壳大黄中),蒽醌(在大黄中)L.)、香豆素(在羌活中)、苯丙烷苷、生物碱和木脂素(在厚朴中),它们构成了三花汤中存在的关键成分。希望本研究对其他中药成分的表征具有一定的意义,为三花汤药理研究奠定基础。
更新日期:2020-07-10
down
wechat
bug