Plants with high biomass can be manipulated for their reducing sugar content which ultimately upon fermentation produces ethanol. This concept was used to enhance the production of reducing sugar from cattail (Typha latifolia) by oxalic acid (OAA) pre-treatment followed by enzymatic saccharification. The optimum condition of total reducing sugar released from OAA pre-treatment was found to be 22.32 mg/ml (OAA—1.2%; substrate concentration (SC)—6%; reaction time (RT)—20 min) using one variable at a time (OVAT). Enzymatic saccharification yielded 45.21 mg/ml of reducing sugar (substrate concentration (SC)—2.4%; enzymatic dosage—50 IU/g; pH 7.0; temp—50 °C) using response surface methodology (RSM). We conclude that Typha can be used as a potential substrate for large-scale biofuel production, employing economical bioprocessing strategies.

中文翻译：

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香蒲中草酸预处理和酶促糖化工艺的优化，用于生产还原糖。

可以对具有高生物量的植物的还原糖含量进行操作，该糖含量最终在发酵后会产生乙醇。通过草酸（OAA）预处理，然后进行酶促糖化，该概念被用于增强香蒲（香蒲（Typha latifolia））的还原糖产量。发现OAA预处理释放的总还原糖的最佳条件是22.32 mg / ml（OAA-1.2％；底物浓度（SC）-6％；反应时间（RT）-20分钟），使用一个变量在时间（OVAT）。使用响应表面方法（RSM）酶促糖化可产生45.21 mg / ml的还原糖（底物浓度（SC）-2.4％；酶促剂量-50 IU / g； pH 7.0；温度-50°C）。我们得出结论，采用经济的生物加工策略，香蒲可以用作大规模生物燃料生产的潜在基质。