The development of microalgal strains for enhanced biomass and biofuel production has received increased attention. Moreover, strain development via metabolic engineering for commercial production is being considered as the most efficient strategy. Pyruvate is an essential metabolite in the cells and plays an essential role in amino acid biosynthesis and de novo fatty acid biosynthesis in plastids. Although pyruvate can be a valuable target for metabolic engineering, its transporters have rarely been studied in microalgae. In this study, we aimed to identify the plastidial pyruvate transporter of Phaeodactylum tricornutum and utilize it for strain development. We identified putative pyruvate transporter localized in the plastid membrane of Phaeodactylum tricornutum. Transformants overexpressing the pyruvate transporter were generated to increase the influx of pyruvate into plastids. Overexpression of a plastidial pyruvate transporter in P. tricornutum resulted in enhanced biomass (13.6% to 21.9%), lipid contents (11% to 30%), and growth (3.3% to 8.0%) compared to those of wild type during one-stage cultivation. To regulate the pyruvate influx and its metabolism in plastids, we generated transformants overexpressing the putative plastidial pyruvate transporter in P. tricornutum. They showed that its overexpression for compartmentalizing pyruvate in plastids could be an attractive strategy for the effective production of biomass and lipids with better growth, via enhanced pyruvate metabolism in plastids.

中文翻译：

---

通过三角褐指藻中推定质体丙酮酸转运蛋白的过度表达增强质体中的丙酮酸代谢。


用于提高生物质和生物燃料生产的微藻菌株的开发受到越来越多的关注。此外，通过代谢工程进行菌株开发用于商业生产被认为是最有效的策略。丙酮酸是细胞中必需的代谢物，在质体中的氨基酸生物合成和脂肪酸从头生物合成中发挥重要作用。尽管丙酮酸可能是代谢工程的一个有价值的靶标，但其转运蛋白很少在微藻中进行研究。在这项研究中，我们的目的是鉴定三角褐指藻的质体丙酮酸转运蛋白，并将其用于菌株开发。我们鉴定了位于三角褐指藻质体膜中的假定丙酮酸转运蛋白。产生过表达丙酮酸转运蛋白的转化体以增加丙酮酸流入质体。与野生型相比，三角藻中质体丙酮酸转运蛋白的过度表达导致生物量（13.6％至21.9％）、脂质含量（11％至30％）和生长（3.3％至8.0％）增加。阶段培养。为了调节丙酮酸流入及其在质体中的代谢，我们产生了在三角藻中过度表达假定的质体丙酮酸转运蛋白的转化体。他们表明，通过增强质体中的丙酮酸代谢，其在质体中分隔丙酮酸的过度表达可能是有效生产生物量和脂质以及更好生长的有吸引力的策略。