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Hepatic Clearance Binding Terms of Hydrophobic Organic Chemicals in Rainbow Trout: Application of a Streamlined Sorbent-Phase Dosing Method
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2020-07-10 , DOI: 10.1021/acs.estlett.0c00518 Leslie J. Saunders 1 , Gabrielle Diaz-Blanco 2 , Yung-Shan Lee 3 , S. Victoria Otton 3 , Frank A. P. C. Gobas 1, 3
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2020-07-10 , DOI: 10.1021/acs.estlett.0c00518 Leslie J. Saunders 1 , Gabrielle Diaz-Blanco 2 , Yung-Shan Lee 3 , S. Victoria Otton 3 , Frank A. P. C. Gobas 1, 3
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Knowledge of the freely dissolved chemical fraction in liver S9 incubation medium (ϕS9) and blood plasma (ϕP), often expressed as the ϕP/ϕS9 ratio or fU, plays a central role in in vitro–in vivo extrapolation of biotransformation rates for hydrophobic organic chemicals (HOCs) in fish. However, there are very few empirical fU data for adequately assessing the bioaccumulation potential of HOCs (i.e., log KOW ≥ 4) from in vitro biotransformation rates. In this study, we present a novel streamlined approach for obtaining the fU values of HOCs and produce some of the first empirical fU data for rainbow trout. We show that fU is relatively constant for substances with a log KOW of ≥4 and appears to be largely controlled by the relative differences in protein and lipid contents in blood plasma and liver S9 incubation media. Predictive fU models are in reasonable agreement with empirical observations. Empirical fU values for HOCs with a log KOW of ≥4 ranged between 0.015 and 0.096 with an average fU value of 0.044 at an S9 protein concentration of 1 mg/mL. This range of fU values may provide reasonable preliminary estimates for the extrapolation of biotransformation rates measured in trout liver S9 bioassays.
中文翻译：
虹鳟鱼中疏水性有机化学物质的肝脏清除结合术语：流线型吸附剂相加药方法的应用
肝S9孵育培养基（ϕ S9）和血浆（ϕ P）中自由溶解的化学成分的知识（通常表示为ϕ P / ϕ S9比率或f U）在体外-体内外推法中起着核心作用。鱼类中疏水有机化学物质的生物转化率。然而，很少有经验˚F ü数据充分评估肝卵圆细胞的生物蓄积潜力（即，登录ķ OW从≥4）体外生物转化率。在这项研究中，我们提出了一种新颖的简化方法来获取f U肝卵圆细胞的值，并产生一些第一经验的˚F ü虹鳟鱼数据。我们显示f U对于log K OW≥4的物质相对恒定，并且似乎主要受血浆和肝脏S9培养介质中蛋白质和脂质含量的相对差异控制。F U的预测模型与经验观察结果是合理一致的。在1 mg / mL的S9蛋白浓度下，log K OW≥4的HOC的经验f U值在0.015至0.096之间，平均f U值为0.044。这个范围fU值可以为在鳟鱼肝脏S9生物测定中测得的生物转化率的推断提供合理的初步估计。
更新日期：2020-09-08
中文翻译：
虹鳟鱼中疏水性有机化学物质的肝脏清除结合术语：流线型吸附剂相加药方法的应用
肝S9孵育培养基（ϕ S9）和血浆（ϕ P）中自由溶解的化学成分的知识（通常表示为ϕ P / ϕ S9比率或f U）在体外-体内外推法中起着核心作用。鱼类中疏水有机化学物质的生物转化率。然而，很少有经验˚F ü数据充分评估肝卵圆细胞的生物蓄积潜力（即，登录ķ OW从≥4）体外生物转化率。在这项研究中，我们提出了一种新颖的简化方法来获取f U肝卵圆细胞的值，并产生一些第一经验的˚F ü虹鳟鱼数据。我们显示f U对于log K OW≥4的物质相对恒定，并且似乎主要受血浆和肝脏S9培养介质中蛋白质和脂质含量的相对差异控制。F U的预测模型与经验观察结果是合理一致的。在1 mg / mL的S9蛋白浓度下，log K OW≥4的HOC的经验f U值在0.015至0.096之间，平均f U值为0.044。这个范围fU值可以为在鳟鱼肝脏S9生物测定中测得的生物转化率的推断提供合理的初步估计。
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