Background. Insulin receptor substrate 1 (IRS-1) is an important molecule of the insulin signal transduction pathway and has been associated with the occurrence and development of many tumors, including hepatocellular carcinoma (HCC). Our study was designed to determine the expression and significance of IRS-1 in human HCC. Methods. Two hundred and forty specimens were drawn from 140 patients, including 100 HCC tissues and 100 paracancerous (PC) tissues from 100 HCC patients, 20 liver cirrhosis (LC) tissues from 20 LC patients, and 20 chronic hepatitis (CH) tissues from 20 CH patients. Baseline and pathological characteristics were included, and the expression of IRS-1 was examined by immunohistochemical (IHC) staining. Binary logistic regression model calculation was used for multivariate analysis. Results. The total positive rates of IRS-1 expression were 41.0%, 17.0%, 15.0%, and 10.0% in HCC, PC, LC and CH tissues, respectively. IRS-1-positive signals were brown in color and located in the nucleus and cytoplasm. Compared with PC, LC, and CH tissues, a significantly increased expression was observed in human HCC tissues (, , and ). Eight of the total 240 specimens had the strong immunostaining of IRS-1 expression, and all of them were HCC tissues. After control of the age, gender, and HBV and HCV infection, IRS-1 expression was independently associated with the diagnosis of HCC (OR 6.60, 95% CI 2.243-19.425, ). Conclusions. Positive expression of IRS-1 in HCC was increased significantly and may play an important role in the occurrence and development of human HCC.

中文翻译：

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胰岛素受体底物1在人肝细胞癌中的表达及意义。

背景。胰岛素受体底物1（IRS-1）是胰岛素信号转导通路的重要分子，与包括肝细胞癌（HCC）在内的多种肿瘤的发生和发展有关。我们的研究旨在确定 IRS-1 在人类 HCC 中的表达和意义。方法。从 140 名患者中抽取了 240 份标本，其中包括来自 100 名 HCC 患者的 100 份 HCC 组织和 100 份癌旁（PC）组织，来自 20 名 LC 患者的 20 份肝硬化（LC）组织，以及来自 20 名 CH 的 20 份慢性肝炎（CH）组织耐心。包括基线和病理学特征，并通过免疫组织化学（IHC）染色检查IRS-1的表达。二元逻辑回归模型计算用于多变量分析。结果。HCC、PC、LC和CH组织中IRS-1表达的总阳性率分别为41.0%、17.0%、15.0%和10.0%。IRS-1 阳性信号呈棕色，位于细胞核和细胞质中。与 PC、LC 和 CH 组织相比，在人 HCC 组织中观察到表达显着增加（, ,和）。总共240个标本中有8个具有IRS-1表达的强免疫染色，并且都是HCC组织。在控制年龄、性别、HBV 和 HCV 感染后，IRS-1 表达与 HCC 的诊断独立相关（OR 6.60，95% CI 2.243-19.425，）。 结论。IRS-1在HCC中的阳性表达显着增加，可能在人HCC的发生发展中起重要作用。