ABSTRACT

Background

Behçet’s disease (BD) is a chronic, multisystemic, inflammatory disease characterized by relapsing episodes of a wide spectrum of clinical findings. The role and mechanism of IFN-λs in BD remain unknown. The aim of this study was to investigate the relationship between IL29 and IL28B gene polymorphisms and BD or clinical manifestations.

Methods

Using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, single-nucleotide polymorphisms of IL28B rs8099917 (IL28 G/T), rs12979860 (IL28 C/T) and IL29 rs30461 (IL29 T/C) were studied in 94 patients with BD and 90 healthy controls.

Results

Our study did not show any relationship between Behçet Disease and genotype or allele frequencies of IL28B (rs8099917, rs12979860) and IL29 (rs30461) gene polymorphisms (p > .05). We found that the TT genotype of rs12979860 (IL28 C/T) polymorphism is higher in healthy controls and patients without central nervous system (CNS) involvement compared to patients with CNS involvement (p = .014 and p = .022).

Conclusions

As a result, although the relationship was found between IL28 and IL29 gene polymorphisms with some clinical manifestations of BD, it was not directly related to the predisposition of the disease. The relationship between IL-28 and IL-29 which act as regulators in inflammatory processes, with Behçet disease, needs to be investigated in further studies.

中文翻译：

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IL28B、IL29基因多态性与白塞氏病临床表现的关系

 抽象的

 背景

白塞氏病 (BD) 是一种慢性、多系统炎症性疾病，其特征是多种临床表现的反复发作。 IFN-λ 在 BD 中的作用和机制仍不清楚。本研究的目的是探讨IL29和IL28B基因多态性与BD或临床表现之间的关系。

 方法

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对IL28B rs8099917（ IL28 G/T）、rs12979860（ IL28 C/T）和IL29 rs30461（ IL29 T/C）的单核苷酸多态性进行研究。 94 名 BD 患者和 90 名健康对照。

 结果

我们的研究没有显示白塞病与IL28B (rs8099917、rs12979860) 和IL29 (rs30461) 基因多态性的基因型或等位基因频率之间存在任何关系 ( p > .05)。我们发现，与 CNS 受累患者相比，健康对照和无中枢神经系统 (CNS) 受累患者的 rs12979860 ( IL28 C/T) 多态性 TT 基因型更高 ( p = .014 和p = .022)。

 结论

结果，虽然发现IL28和IL29基因多态性与BD的一些临床表现之间存在关系，但与疾病的易感性并无直接关系。 IL-28 和 IL-29 作为炎症过程的调节因子，与白塞病之间的关系需要进一步研究。