Pediococcus pentosaceus, a bacterium recently used in human and animal probiotics, was used in combination with supports made from Polylactic acid (PLA) composite soybean meal was usedto study biofilm formation, and it was found that dense biofilms developed by Day 1. Proteomic comparison between planktonic and biofilm cultures of P. pentosaceus showed distinct expression patterns of intracellular and extracellular proteins. Type I glyceraldehyde-3-phosphate dehydrogenase was upregulated in biofilm cultures and mediated cell adhesion and encouraged biofilm production. GMP synthase, which regulates GMP synthesis and acts as an intracellular signal molecule to control cell mechanisms and has been exploited in the development of new therapeutic agents, was also upregulated in the biofilm mode of growth. The present work serves as a basis for future studies examining the complex network of systems that regulate Lactic Acid Bacterial (LAB) biofilm formation and can serve as a framework for studies of production of therapeutic agents from LAB.

中文翻译：

---

戊糖片球菌生物膜的生理学研究

戊糖片球菌，一种最近用于人类和动物益生菌的细菌，与由聚乳酸 (PLA) 复合豆粕制成的支持物结合用于研究生物膜形成，发现第 1 天形成致密的生物膜。 蛋白质组学比较P. pentosaceus 的浮游和生物膜培养物显示出不同的细胞内和细胞外蛋白表达模式。I型甘油醛-3-磷酸脱氢酶在生物膜培养物中上调并介导细胞粘附并促进生物膜的产生。GMP 合酶调节 GMP 合成并作为细胞内信号分子来控制细胞机制，并已被用于开发新的治疗剂，在生物膜生长模式中也被上调。