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IL-31 transgenic mice show reduced allergen-induced lung inflammation.
European Journal of Immunology ( IF 4.5 ) Pub Date : 2020-07-10 , DOI: 10.1002/eji.202048547
Theresa Neuper 1 , Daniel Neureiter 2 , Muamera Sarajlic 1 , Helen Strandt 1 , Renate Bauer 1 , Harald Schwarz 1 , Patrick Suchanek 1 , Evgeniia Korotchenko 1 , Stacey R Dillon 3 , Peter Hammerl 1 , Angelika Stoecklinger 1 , Richard Weiss 1 , Jutta Horejs-Hoeck 1
Affiliation  

Interleukin‐31 (IL‐31) is a Th2 cell–derived cytokine that has been closely linked to pruritic skin inflammation. More recently, enhanced IL‐31 serum levels have also been observed in patients with allergic rhinitis and allergic asthma. Therefore, the main aim of this study was to unravel the contribution of IL‐31 to allergen‐induced lung inflammation. We analyzed lung inflammation in response to the timothy grass (Phleum pratense) pollen allergen Phl p 5 in C57BL/6 wild‐type (wt) mice, IL‐31 transgenic (IL‐31tg) mice, and IL‐31 receptor alpha‐deficient animals (IL‐31RA−/−). IL‐31 and IL‐31RA levels were monitored by qRT‐PCR. Cellular infiltrate in bronchoalveolar lavage fluid (BALF) and lung tissue inflammation, mucus production as well as epithelial thickness were measured by flow cytometry and histomorphology. While allergen challenge induced IL‐31RA expression in lung tissue of wt and IL‐31tg mice, high IL‐31 expression was exclusively observed in lung tissue of IL‐31tg mice. Upon Phl p 5 challenge, IL‐31tg mice showed reduced numbers of leukocytes and eosinophils in BALF and lung tissue as well as diminished mucin expression and less pronounced epithelial thickening compared to IL‐31RA−/− or wt animals. These findings suggest that the IL‐31/IL‐31RA axis may regulate local, allergen‐induced inflammation in the lungs.

中文翻译:

IL-31 转基因小鼠表现出过敏原诱导的肺部炎症减少。

白细胞介素-31 (IL-31) 是一种 Th2 细胞衍生的细胞因子,与瘙痒性皮肤炎症密切相关。最近,在过敏性鼻炎和过敏性哮喘患者中也观察到 IL-31 血清水平升高。因此,本研究的主要目的是阐明 IL-31 对过敏原诱导的肺部炎症的作用。我们分析了 C57BL/6 野生型 (wt) 小鼠、IL-31 转基因 (IL-31tg) 小鼠和 IL-31 受体 α 缺陷小鼠对梯牧草 ( Phleum pratense ) 花粉过敏原 Phl p 5的肺部炎症反应。动物(IL-31RA -/-)。通过 qRT-PCR 监测 IL-31 和 IL-31RA 水平。通过流式细胞术和组织形态学测量支气管肺泡灌洗液(BALF)中的细胞浸润和肺组织炎症、粘液产生以及上皮厚度。虽然过敏原攻击诱导 wt 和 IL-31tg 小鼠肺组织中 IL-31RA 表达,但仅在 IL-31tg 小鼠肺组织中观察到高 IL-31 表达。在 Phl p 5 攻击后,与 IL-31RA −/−或 wt 动物相比,IL-31tg 小鼠的 BALF 和肺组织中白细胞和嗜酸性粒细胞数量减少,粘蛋白表达减少,上皮增厚不那么明显。这些发现表明 IL-31/IL-31RA 轴可能调节局部过敏原诱导的肺部炎症。
更新日期:2020-07-10
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