Cytoplasmic polyadenylation element‐binding protein 3 (CPEB3) is a member of the Cytoplasmic polyadenylation element‐binding family, which has been found to regulate the translation of dormant and masked mRNA in Xenopus oocytes and plays potential roles in regulating biological functions in cells and tissues. However, its role in cumulus cells is not clear. In this study, the mRNA expression of CPEB3 in bovine cumulus cells was inhibited with small interfering RNA. Cell cycle progression, proliferation, and apoptosis were measured after inhibition of CPEB3. Subsequently, changes in intracellular Reactive oxygen species content, mitochondrial membrane potential and expansion‐related gene expression were examined. The results showed that after CPEB3 inhibition, cumulus cells had an abnormal cell cycle, the numbers of cells in the S and G2/M phases were significantly increased, cell proliferation was increased and apoptosis rates were decreased. These effects were likely due CPEB3 inhibition‐induced decreases in intracellular Reactive oxygen species levels; increases in mitochondrial membrane potential; decreases in apoptosis; downregulation of CCNA, CCND, CCNE, CDK2, CDK4, CDK6, p21, and p27 mRNA expression; and upregulation of CCNB, CDK1, HAS2, PTGS2, PTX3, and CEBPB mRNA expression. Therefore, CPEB3 plays potential roles in regulating the biological and physiological functions of bovine cumulus cell.

中文翻译：

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CPEB3 调节牛卵丘细胞的增殖和凋亡。

细胞质聚腺苷酸化元件结合蛋白 3 (CPEB3) 是细胞质聚腺苷酸化元件结合家族的成员，已被发现可调节非洲爪蟾卵母细胞中休眠和掩蔽 mRNA 的翻译，并在调节细胞和组织的生物学功能中发挥潜在作用. 然而，其在卵丘细胞中的作用尚不清楚。在这项研究中，CPEB3 在牛卵丘细胞中的 mRNA 表达被小干扰 RNA 抑制。抑制 CPEB3 后测量细胞周期进程、增殖和凋亡。随后，检查了细胞内活性氧含量、线粒体膜电位和扩增相关基因表达的变化。结果表明，CPEB3抑制后，卵丘细胞出现细胞周期异常，S期和G2/M期细胞数量显着增加，细胞增殖增加，凋亡率降低。这些影响可能是由于 CPEB3 抑制导致细胞内活性氧水平降低；线粒体膜电位增加；细胞凋亡减少；CCNA、CCND、CCNE、CDK2、CDK4、CDK6、p21 和 p27 mRNA 表达的下调；CCNB、CDK1、HAS2、PTGS2、PTX3 和 CEBPB mRNA 表达的上调。因此，CPEB3在调节牛卵丘细胞的生物学和生理功能方面具有潜在的作用。线粒体膜电位增加；细胞凋亡减少；CCNA、CCND、CCNE、CDK2、CDK4、CDK6、p21 和 p27 mRNA 表达的下调；CCNB、CDK1、HAS2、PTGS2、PTX3 和 CEBPB mRNA 表达的上调。因此，CPEB3在调节牛卵丘细胞的生物学和生理功能方面具有潜在的作用。线粒体膜电位增加；细胞凋亡减少；CCNA、CCND、CCNE、CDK2、CDK4、CDK6、p21 和 p27 mRNA 表达的下调；CCNB、CDK1、HAS2、PTGS2、PTX3 和 CEBPB mRNA 表达的上调。因此，CPEB3在调节牛卵丘细胞的生物学和生理功能方面具有潜在的作用。