Ripe rot is one of the most important bunch diseases of grapes in the highlands of southern Brazil and a matter of concern for winegrowers. Sixty‐one strains isolated from berries of Vitis labrusca and V. vinifera varieties with symptoms were classified by phylogenetic analysis of the sequences of β‐tubulin, glyceraldehyde‐3‐phosphate dehydrogenase, D1/D2 domain of 28S rDNA, and rDNA internal transcribed spacer. They were also characterized by morphology, and their pathogenicity was evaluated. The combined molecular data allowed identification of six Colletotrichum species: C. fructicola, C. kahawae and C. viniferum (gloeosporioides complex), C. limitticola, C. nymphaeae (acutatum complex), and C. karstii (boninense complex). This is the first report of C. karstii and C. limitticola associated with the ripe rot of grapes. Morphological characteristics varied within and among species, confirming their separation at the complex level. Pathogenicity tests on V. vinifera berries showed that the most prevalent species, C. viniferum (37.8%) and C. fructicola (36.1%), were more virulent than the less prevalent species C. limitticola and C. karstii. Our findings indicate that there is a high diversity of Colletotrichum species associated with ripe rot disease of grapes in Brazil. There were no clear differences in the distribution of Colletotrichum species between V. labrusca and V. vinifera varieties. The determination of fungal species responsible for grape ripe rot in Brazilian vineyards may contribute to further epidemiological studies and the development of more efficient prophylactic methods for ripe rot management.

中文翻译：

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在巴西南部高地的Vitis labrusca和V. vinifera品种中引起葡萄成熟腐烂病的炭疽菌物种

成熟腐烂是巴西南部高原上最重要的葡萄串病之一，也是葡萄种植者关注的问题。从Vitis labrusca和V的浆果中分离出六十一株。通过系统发育分析β-微管蛋白，甘油醛-3-磷酸脱氢酶，28S rDNA的D1 / D2结构域和rDNA内部转录间隔区的序列，对具有症状的葡萄品种进行分类。它们还通过形态学表征，并评估其致病性。将合并的分子数据允许六个识别炭疽病物种：Ç。苦瓜，C。kahawae和C。viniferum（gloeosporioides complex），C。Limitticola，C . nymphaeae（acutatum复合物），和Ç。karstii（boninense complex）。这是C的第一个报告。karstii和Ç。与葡萄腐烂相关的极限。物种内部和物种之间的形态特征各不相同，从而证实了它们在复杂水平上的分离。V的致病性测试。葡萄浆果表明，最普遍的品种，Ç。葡萄（37.8％）和C。毛果（36.1％）比不那么普遍的物种C更具毒性。limitticola和Ç。卡尔斯蒂。我们的发现表明，在巴西，与葡萄成熟腐烂病相关的炭疽菌种类非常多样。V之间炭疽菌种类的分布没有明显差异。labrusca和V。葡萄品种。确定巴西葡萄园葡萄成熟腐烂的真菌种类可能有助于进一步的流行病学研究，并为成熟腐烂管理开发更有效的预防方法。